Qi Wenbo, Yang Zhaoting, Li Haoyue, Cui Yan, Xuan Yanhua
Key Laboratory of Natural Resources of the Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Yanji, China.
Department of Pathology, Yanbian University College of Medicine, Yanji, China.
APMIS. 2019 Feb;127(2):64-71. doi: 10.1111/apm.12919.
The aim of the present study was to identify the relation between Tenascin-C (TNC) and Twist1 and determine their clinical significance in gastric cancer (GC). We analyzed the expression of TNC and Twist1 in 159 GC samples and in 91 non-tumor samples using immunohistochemistry. In this study, TNC expression in stromal fibroblasts of GC was remarkably higher than non-tumor gastric lesions. The expression of TNC in GC stromal fibroblasts was significantly associated with pT stage, lymph node metastasis, distant metastasis. Twist1 expression in stromal fibroblasts of GC was remarkably higher than non-tumor gastric lesions. Twist1 expression in the stromal fibroblasts of GC was associated with tumor size, lymph node metastasis, and clinical stage. Furthermore, TNC expression levels in GC stromal fibroblasts were positively associated with Twist1. The simultaneous expression of TNC and Twist1 was significantly higher in stromal fibroblasts of GC than in noncancerous tissues. The simultaneous expression of TNC and Twist1 in GC stromal fibroblasts was positively associated with tumor location, pT stage, lymph node metastasis and clinical stage. Moreover, patients with co-expression of TNC and Twist1 had a poorer prognosis than either TNC or Twist1 positive in GC. Our study revealed that the simultaneous expression of TNC and Twist1 indicated the poorer prognosis of GC. Co-targeting TNC and Twist1 confer significant clinical advantage, which offers a novel therapeutic target in GC.
本研究的目的是确定肌腱蛋白-C（TNC）与Twist1之间的关系，并确定它们在胃癌（GC）中的临床意义。我们采用免疫组织化学方法分析了159例GC样本和91例非肿瘤样本中TNC和Twist1的表达情况。在本研究中，GC间质成纤维细胞中TNC的表达明显高于非肿瘤性胃病变。GC间质成纤维细胞中TNC的表达与pT分期、淋巴结转移、远处转移显著相关。GC间质成纤维细胞中Twist1的表达明显高于非肿瘤性胃病变。GC间质成纤维细胞中Twist1的表达与肿瘤大小、淋巴结转移及临床分期相关。此外，GC间质成纤维细胞中TNC的表达水平与Twist1呈正相关。GC间质成纤维细胞中TNC和Twist1的同时表达明显高于非癌组织。GC间质成纤维细胞中TNC和Twist1的同时表达与肿瘤位置、pT分期、淋巴结转移及临床分期呈正相关。此外，GC中TNC和Twist1共表达的患者预后比TNC或Twist1单一阳性的患者更差。我们的研究表明，TNC和Twist1的同时表达提示GC预后较差。同时靶向TNC和Twist1具有显著的临床优势，为GC提供了一个新的治疗靶点。
