Ni Wei-Dong, Yang Zhao-Ting, Cui Chun-Ai, Cui Yan, Fang Long-Yun, Xuan Yan-Hua
Key Laboratory of Natural Resources of the Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Yanji 133002, China; Institute for Regenerative Medicine, Yanbian University College of Medicine, Yanji 133002, China.
Institute for Regenerative Medicine, Yanbian University College of Medicine, Yanji 133002, China.
Biochem Biophys Res Commun. 2017 May 6;486(3):607-612. doi: 10.1016/j.bbrc.2017.03.021. Epub 2017 Mar 21.
Tenascin-C (TNC), as a member of the extracellular matrix (ECM), plays an important role in cancer cell proliferation and migration and tumor invasion in various types of cancer. Here, we attempted to investigate the role of TNC as a prognostic factor in prostate cancer. We studied TNC expression via immunohistochemistry in 145 prostate cancer tissue samples. The clinicopathological relevance of TNC expression was examined, as well as other cancer-associated fibroblasts (CAFs)-related factors. Our results showed that the high levels of TNC expression in prostate cancer stroma was significantly associated with lymph node metastasis (P = 0.024) and clinical stage (P = 0.032). Furthermore, TNC was positively correlated with increased micro-vessel density (MVD) (P = 0.017) and tumor associated macrophage (TAM) population (P = 0.025). In both univariate and multivariate Cox regression analyses, TNC (P < 0.001) was an independent poor prognostic factor for overall survival in prostate cancer patients. Moreover, over-expression of TNC (P < 0.001), SMA (P = 0.042) and vimentin (P = 0.010) were significantly correlated with the lower overall survival. In addition, TNC expression in prostate cancer stroma was significantly associated with FSP1 (P = 0.011), SMA (P = 0.021), and vimentin (P = 0.002). In conclusion, our study revealed that high level of TNC as a potential biomarker of CAFs was significantly correlated with the poor prognosis for prostate cancer patients.
腱生蛋白-C（TNC）作为细胞外基质（ECM）的一员，在各类癌症的癌细胞增殖、迁移及肿瘤侵袭过程中发挥着重要作用。在此，我们试图探究TNC作为前列腺癌预后因素的作用。我们通过免疫组织化学研究了145例前列腺癌组织样本中TNC的表达情况。检测了TNC表达与临床病理的相关性，以及其他与癌症相关成纤维细胞（CAFs）相关的因素。我们的结果显示，前列腺癌基质中TNC的高表达与淋巴结转移（P = 0.024）及临床分期（P = 0.032）显著相关。此外，TNC与微血管密度（MVD）增加（P = 0.017）及肿瘤相关巨噬细胞（TAM）数量（P = 0.025）呈正相关。在单因素和多因素Cox回归分析中，TNC（P < 0.001）是前列腺癌患者总生存期的独立不良预后因素。此外，TNC（P < 0.001）、平滑肌肌动蛋白（SMA，P = 0.042）和波形蛋白（P = 0.010）的过表达与较低的总生存期显著相关。另外，前列腺癌基质中TNC的表达与FSP1（P = 0.011）、SMA（P = 0.021）和波形蛋白（P = 0.002）显著相关。总之，我们的研究表明，高水平的TNC作为CAFs的潜在生物标志物与前列腺癌患者的不良预后显著相关。
