使用¹⁴C标记底物研究2-(3-甲基肉桂酰肼基)丙酸酯对离体大鼠膈肌脂肪酸和葡萄糖氧化的影响。酰肼基丙酸类，一类新型降血糖物质，VIII。

Effects of 2-(3-methyl-cinnamyl-hydrazono)-propionate on fatty acid and glucose oxidation in the isolated rat diaphragm using 14C-labelled substrates. Hydrazonopropionic acids, a new class of hypoglycaemic substances, VIII.

作者信息

Binder L, Oellerich M, Haeckel R, Beneking M

机构信息

Institut für Klinische Chemie, Medizinische Hochschule Hannover, Federal Republic of Germany.

出版信息

J Clin Chem Clin Biochem. 1988 Dec;26(12):815-9. doi: 10.1515/cclm.1988.26.12.815.

DOI:10.1515/cclm.1988.26.12.815

PMID:3069950

Abstract

The influence of 2-(3-methyl-cinnamyl-hydrazono)-propionate on the utilization of various substrates in isolated rat hemidiaphragms was investigated in comparison with other hypoglycaemic compounds. The effect of 2-(3-methyl-cinnamyl-hydrazono)-propionate was concentration-dependent. At a concentration of 0.5 mmol/l 2-(3-methyl-cinnamyl-hydrazono)-propionate, glucose utilization increased from 0.276 +/- 0.043 mumol.g-1.l-1 to 0.894 +/- 0.303 mumol.g-1.l-1 (p less than 0.05). Pyruvate and lactate utilization were stimulated to a lesser extent, while acetate utilization remained nearly constant. At a concentration of 2 mmol/l 2-(3-methyl-cinnamyl-hydrazono)-propionate, the oxidation of palmitate decreased from 0.214 +/- 0.017 mumol.g-1.l-1 to 0.060 +/- 0.005 mumol.g-1.l-1, while the oxidation of octanoate was not decreased. These findings point to a stimulation of the glycolytic flux by inhibition of long-chain fatty acid oxidation.

摘要

与其他降血糖化合物相比，研究了2-(3-甲基肉桂酰肼基)丙酸酯对离体大鼠半膈肌中各种底物利用的影响。2-(3-甲基肉桂酰肼基)丙酸酯的作用呈浓度依赖性。在浓度为0.5 mmol/l的2-(3-甲基肉桂酰肼基)丙酸酯作用下，葡萄糖利用率从0.276±0.043 μmol·g-1·l-1增加到0.894±0.303 μmol·g-1·l-1（p<0.05）。丙酮酸和乳酸的利用率受到的刺激较小，而乙酸的利用率几乎保持不变。在浓度为2 mmol/l的2-(3-甲基肉桂酰肼基)丙酸酯作用下，棕榈酸的氧化从0.214±0.017 μmol·g-1·l-1降至0.060±0.005 μmol·g-1·l-1，而辛酸的氧化未降低。这些发现表明，通过抑制长链脂肪酸氧化可刺激糖酵解通量。