Babu Talluri Kiran, Sharma Rajan, Thakur R P, Upadhyaya Hari D, Reddy P Narayan, Girish A G
Acharya N. G. Ranga Agricultural University (ANGRAU), Rajendranagar, Hyderabad 500030, Telangana, India; and International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad 502324, Telangana, India.
ICRISAT, Telangana, India.
Plant Dis. 2015 Dec;99(12):1784-1789. doi: 10.1094/PDIS-10-14-1089-RE. Epub 2015 Oct 4.
Blast, caused by Pyricularia grisea (teleomorph: Magnaporthe grisea), is the most devastating disease of finger millet affecting production, utilization, and trade in Africa and Southeast Asia. An attempt was made to select a set of putative host differentials that can be used to determine virulence diversity in finger-millet-infecting populations of M. grisea. Thus, a differential set comprising eight germplasm accessions selected from finger millet core collection (IE 2911, IE 2957, IE 3392, IE 4497, IE 5091, IE 6240, IE 6337, and IE 7079) and a resistant ('GPU 28') and a susceptible ('VR 708') variety was developed. This differential set was used to study pathogenic variation in 25 isolates of M. grisea collected from Karnataka, Telangana, and Andhra Pradesh states in India. Based on the reaction (virulent = score ≥4 and avirulent = score ≤3 on a 1-to-9 scale) on host differentials, nine pathotypes were identified among 25 M. grisea isolates. Pathotype 9, represented by isolate Pg23 from Vizianagaram, was the most virulent because it could infect all of the host differentials except GPU 28. This study will be helpful in devising strategies for monitoring virulence change in M. grisea populations, and for identification of blast resistance in finger millet for use in disease resistance breeding programs.
稻瘟病由稻瘟病菌（有性型：灰梨孢菌）引起，是影响非洲和东南亚地区黍稷生产、利用和贸易的最具毁灭性的病害。人们试图挑选一组假定的鉴别寄主，用于确定侵染黍稷的稻瘟病菌群体中的毒力多样性。因此，构建了一个鉴别寄主组合，包括从黍稷核心种质库中选出的8份种质材料（IE 2911、IE 2957、IE 3392、IE 4497、IE 5091、IE 6240、IE 6337和IE 7079）以及一个抗病品种（‘GPU 28’）和一个感病品种（‘VR 708’）。利用该鉴别寄主组合研究了从印度卡纳塔克邦、特伦甘纳邦和安得拉邦采集的25株稻瘟病菌的致病变异情况。根据对鉴别寄主的反应（在1至9级评分标准上，强毒力=评分≥4，无毒力=评分≤3），在25株稻瘟病菌分离株中鉴定出9种致病型。以来自维扎加帕拉姆的分离株Pg23为代表的致病型9毒力最强，因为它能侵染除GPU 28之外的所有鉴别寄主。本研究将有助于制定监测稻瘟病菌群体毒力变化的策略，以及鉴定黍稷中的稻瘟病抗性，用于抗病育种计划。
