Tang L, Gao Z G, Yao Y, Liu X
Plant Protection College, Shenyang Agricultural University, Shenyang, China 110866, and Life Science College, Luoyang Normal University, Luoyang, China 471000.
Plant Protection College.
Plant Dis. 2015 Apr;99(4):482-487. doi: 10.1094/PDIS-06-14-0570-RE.
Setosphaeria turcica is an important pathogen that causes northern leaf blight of corn and sorghum. In total, 188 isolates were isolated from diseased leaves of maize and sorghum in China. However, differences in morphology were not apparent among S. turcica isolates derived from different hosts. The formae speciales were identified as S. turcica. f. sp. zeae and S. turcica. f. sp. sorghi. Universally primed polymerase chain reaction (UP-PCR) analysis led to the conclusion that genetic differences were present among these formae speciales and divided these isolates into five clades. The isolates of each clade belonged to the same forma specialis, and different formae speciales were assigned to different clades. The results of the present study demonstrated that pathogenic specialization of the S. turcica isolate correlated more closely with genetic diversity than geographical distribution. Therefore, combined with assays for pathogenicity, UP-PCR analysis yields an enhanced understanding of the formae speciales of S. turcica.
玉米大斑病菌是引起玉米和高粱北方叶斑病的一种重要病原菌。从中国的玉米和高粱病叶中共分离出188个菌株。然而，来源于不同寄主的玉米大斑病菌株在形态上并无明显差异。这些专化型被鉴定为玉米大斑病菌玉米专化型和玉米大斑病菌高粱专化型。通用引物聚合酶链反应（UP-PCR）分析得出结论，这些专化型之间存在遗传差异，并将这些菌株分为五个分支。每个分支的菌株属于同一专化型，不同的专化型被分配到不同的分支。本研究结果表明，玉米大斑病菌株的致病专化性与遗传多样性的相关性比与地理分布的相关性更为密切。因此，结合致病性测定，UP-PCR分析能更深入地了解玉米大斑病菌的专化型。
