Alonso M J, De Arriaga D, Soler J
Departamento de Bioquímica y Biología Molecular Universidad de León, Spain.
J Enzyme Inhib. 1988;2(2):143-50. doi: 10.3109/14756368809040719.
We have partially characterized an intracellular fraction from Phycomyces blakesleeanus which shows proteolytic activity. The apparent thermal inactivation constant (Kd) was 0.12 min-1 at 50 degrees C. This proteolytic fraction was split into two active fractions by ultrafiltration using a membrane with an exclusion size of 30,000. Both fractions were inhibited by phenyl methyl sulphonyl fluoride. The Ki value for the fraction with molecular weight greater than 30,000 was 0.075 mM. The fraction with molecular weight less than 30,000 inactivated the Phycomyces CPS.
我们已经对来自布氏根霉的显示蛋白水解活性的细胞内部分进行了部分表征。在50摄氏度时，表观热失活常数（Kd）为0.12分钟⁻¹。使用截留分子量为30,000的膜通过超滤将该蛋白水解部分分成两个活性部分。两个部分均被苯甲基磺酰氟抑制。分子量大于30,000的部分的Ki值为0.075 mM。分子量小于30,000的部分使根霉CPS失活。
