关于高峰淀粉酶A底物特异性的研究。十三。6-脱氧-6-碘麦芽寡糖的制备及其对高峰淀粉酶A1的抑制作用。

Studies on the substrate specificity of Taka-amylase A. XIII. Preparation of 6-deoxy-6-iodomaltooligosaccharides and their inhibitory action against Taka-amylase A1.

作者信息

Omichi K, Fujii K, Mizukami T, Matsushima Y

出版信息

J Biochem. 1978 May;83(5):1443-7. doi: 10.1093/oxfordjournals.jbchem.a132054.

DOI:10.1093/oxfordjournals.jbchem.a132054

Abstract

O-alpha-D-Glucopyranosyl-(1 leads to 4)-O-6-deoxy-6-iodo-alpha-D-glucopyranosyl-(1 leads to 4)-D-glucopyranose (6'-MT), O-alpha-D-glucopyranosyl-(1 leads to 4)-6-deoxy-6-iodo-D-glucopyranose (6-M), and O-6-deoxy-6-iodo-alpha-D-glucopyranosyl-(1 leads to 4)-D-glucopyranose (6'-M) were prepared and their inhibitory action against Taka-amylase A [EC 3.2.1.1, alpha-1, 4-glucan 4-glucanohydrolase, Aspergillus oryzae] was investigated. The inhibitor constants of 6'-MT and 6'-M were 10 mM and 54 mM, respectively, and both inhibitors showed mixed-type inhibition. 6-M scarcely inhibited the enzyme action.

摘要

制备了O-α-D-吡喃葡萄糖基-(1→4)-O-6-脱氧-6-碘-α-D-吡喃葡萄糖基-(1→4)-D-吡喃葡萄糖(6'-MT)、O-α-D-吡喃葡萄糖基-(1→4)-6-脱氧-6-碘-D-吡喃葡萄糖(6-M)和O-6-脱氧-6-碘-α-D-吡喃葡萄糖基-(1→4)-D-吡喃葡萄糖(6'-M)，并研究了它们对米曲霉α-淀粉酶A[EC 3.2.1.1，α-1,4-葡聚糖4-葡聚糖水解酶]的抑制作用。6'-MT和6'-M的抑制常数分别为10 mM和54 mM，两种抑制剂均表现为混合型抑制。6-M几乎不抑制该酶的活性。