Organ Transplant Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, PR China.
Guangdong Provincial Key Laboratory of Organ Donation & Transplant Immunology, Guangzhou 510080, PR China.
Epigenomics. 2019 Apr;11(5):527-542. doi: 10.2217/epi-2018-0189. Epub 2019 Jan 31.
To explore molecular mechanisms underlying liver ischemia-reperfusion injury (IRI).
MATERIALS & METHODS: Four Gene Expression Omnibus datasets comprising liver transplantation data were collected for a comprehensive analysis. A proteomic analysis was performed and used for correlations analysis with transcriptomic.
RESULTS & CONCLUSION: Ten differentially expressed genes were co-upregulated in four Gene Expression Omnibus datasets, including ATF3, CCL4, DNAJB1, DUSP5, JUND, KLF6, NFKBIA, PLAUR, PPP1R15A and TNFAIP3. The combined analysis demonstrated ten coregulated genes/proteins, including HBB, HBG2, CA1, SLC4A1, PLIN2, JUNB, HBA1, MMP9, SLC2A1 and PADI4. The coregulated differentially expressed genes and coregulated genes/proteins formed a tight interaction network and could serve as the core factors underlying IRI. Comprehensive and combined omics analyses revealed key factors underlying liver IRI, and thus having potential clinical significance.
探讨肝缺血再灌注损伤（IRI）的分子机制。
收集了四个包含肝移植数据的基因表达综合数据集，进行全面分析。进行了蛋白质组学分析，并与转录组学进行相关性分析。
在四个基因表达综合数据集中共检测到 10 个差异表达基因上调，包括 ATF3、CCL4、DNAJB1、DUSP5、JUND、KLF6、NFKBIA、PLAUR、PPP1R15A 和 TNFAIP3。联合分析表明，有 10 个核心调控基因/蛋白，包括 HBB、HBG2、CA1、SLC4A1、PLIN2、JUNB、HBA1、MMP9、SLC2A1 和 PADI4。这些共同调控的差异表达基因和共同调控的基因/蛋白形成了一个紧密的相互作用网络，可能是 IRI 的核心因素。全面和综合的组学分析揭示了肝 IRI 的关键因素，因此具有潜在的临床意义。
