Inada K
Department of Ophthalmology, Kumamoto University Medical School, Japan.
Jpn J Ophthalmol. 1988;32(4):438-43.
Although the aqueous humor contained several serum proteins and the concentration of each protein was very low in normal eyes, the aqueous/serum ratio of prealbumin was reported to be higher than the ratios of other human aqueous proteins in a previous paper. This study investigated the possibility of the intraocular synthesis of prealbumin, by examining postmortem human eyes using the peroxidase-antiperoxidase method. The immunohistochemical survey revealed the presence of prealbumin in the nonpigmented layer of the ciliary epithelium and in the ganglion cell layer of the retina. Some cells situated on the inner side of the internal nuclear layer of the retina also showed staining. We believe that this is the first study to demonstrate the possibility of synthesis of prealbumin in the human eye by immunohistochemistry.
尽管房水中含有几种血清蛋白，且在正常眼中每种蛋白的浓度都非常低，但在之前的一篇论文中报道，前白蛋白的房水/血清比值高于其他人类房水蛋白的比值。本研究通过使用过氧化物酶-抗过氧化物酶方法检查死后的人眼，来研究前白蛋白眼内合成的可能性。免疫组织化学调查显示，睫状体上皮的无色素层和视网膜的神经节细胞层中存在前白蛋白。视网膜内核层内侧的一些细胞也显示出染色。我们认为，这是第一项通过免疫组织化学证明人眼中前白蛋白合成可能性的研究。
