Férard Jean François, Pérez Karen F Burga, Blaise Christian, Péry Alexandre, Sutthivaiyakit Pakawadee, Gagné François
LIEC Laboratory, University of Lorraine, Metz, France.
Environment Canada, Montréal, Québec, Canada.
J Xenobiot. 2015 Jun 9;5(1):5125. doi: 10.4081/xeno.2015.5125. eCollection 2015 Apr 29.
The ecotoxic potential of seven Moselle river watershed sediments was assessed with a battery of bioassays comprised of rapid phototrophic [LuminoTox solid phase (L-SPA) and elutriate (L-ELU) assays] and bacterial [Microtox solid phase assay (M-SPA)] exposure tests, as well as with two micro-invertebrate solid phase tests conducted with (lethal and sublethal effects solid phase assay, H-SPA and H-SPA) and . Measured effects of sediments and their elutriates were varied and reflected responses that were ecotoxicity test-, endpoint- and site-dependent, suggesting some degree of risk toward benthic and water column organisms, respectively, at specific sites. Correlation analysis demonstrated that L-SPA and M-SPA ecotoxicity responses were significantly linked with the Hydra H-SPA assay, indicating their ability to predict ecotoxicity towards an invertebrate taxonomic group representing secondary consumers. While the L-SPA and M-SPA assays hold promise as rapid screens for sediment ecotoxicity, correlation analysis with grain size (L-SPA: r=-0.795, P=0.033; M-SPA: r=-0.73, P=0.07) points out that their responses can be influenced by the presence of fines (., sediment particles ≤0.063 mm in size) and that this information is essential to properly interpret ecotoxicity data generated with these assays. Finally, notable differences observed in trophic level sensitivities once again recall the importance of employing a test battery to adequately appraise the ecotoxicity of sediments.
通过一系列生物测定评估了摩泽尔河流域七条河流沉积物的生态毒性潜力,这些生物测定包括快速光养生物测定(LuminoTox 固相测定法(L-SPA)和淘析液测定法(L-ELU))、细菌测定(Microtox 固相测定法(M-SPA))暴露试验,以及使用水螅进行的两项微型无脊椎动物固相试验(致死和亚致死效应固相测定法,H-SPA 和 H-SPA)和使用[具体生物名称未给出]进行的试验。沉积物及其淘析液的测量效应各不相同,反映出的反应因生态毒性试验、终点和地点而异,表明在特定地点分别对底栖生物和水柱生物存在一定程度的风险。相关性分析表明,L-SPA 和 M-SPA 的生态毒性反应与水螅 H-SPA 测定法显著相关,表明它们能够预测对代表二级消费者的无脊椎动物分类群的生态毒性。虽然 L-SPA 和 M-SPA 测定法有望作为沉积物生态毒性的快速筛选方法,但与粒度的相关性分析(L-SPA:r = -0.795,P = 0.033;M-SPA:r = -0.73,P = 0.07)指出,它们的反应可能受细颗粒(即尺寸≤0.063 毫米的沉积物颗粒)的影响,并且此信息对于正确解释这些测定法产生的生态毒性数据至关重要。最后,在营养级敏感性方面观察到的显著差异再次提醒人们采用一套试验来充分评估沉积物生态毒性的重要性。