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Upregulation of endothelial gene markers in Wharton's jelly mesenchymal stem cells cultured on polyelectrolyte multilayers.在聚电解质多层膜上培养的脐带华通氏胶间充质干细胞中内皮基因标志物的上调。
J Biomed Mater Res A. 2017 Jan;105(1):292-300. doi: 10.1002/jbm.a.35868. Epub 2016 Oct 31.
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Hyaluronan: A modulator of the tumor microenvironment.透明质酸：肿瘤微环境的调节因子。
Cancer Lett. 2016 May 28;375(1):20-30. doi: 10.1016/j.canlet.2016.02.031. Epub 2016 Feb 24.
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Chitosan and Its Potential Use as a Scaffold for Tissue Engineering in Regenerative Medicine.壳聚糖及其在再生医学中作为组织工程支架的潜在用途。
Biomed Res Int. 2015;2015:821279. doi: 10.1155/2015/821279. Epub 2015 Oct 4.
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Polyelectrolyte Films Boost Progenitor Cell Differentiation into Endothelium-like Monolayers.聚电解质膜促进祖细胞向内皮样单层细胞分化。
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Polyelectrolyte Multilayer Assemblies on Materials Surfaces: From Cell Adhesion to Tissue Engineering.材料表面的聚电解质多层组装体：从细胞黏附到组织工程
Chem Mater. 2012 Mar 13;24(5):854-869. doi: 10.1021/cm2032459.
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Chitosan-based scaffolds for bone tissue engineering.用于骨组织工程的壳聚糖基支架
J Mater Chem B. 2014 Jun 7;2(21):3161-3184. doi: 10.1039/C4TB00027G.
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Materials as stem cell regulators.材料作为干细胞调控物。
Nat Mater. 2014 Jun;13(6):547-57. doi: 10.1038/nmat3937.
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Natural and synthetic biodegradable polymers: different scaffolds for cell expansion and tissue formation.天然和合成可生物降解聚合物：用于细胞扩增和组织形成的不同支架。
Int J Artif Organs. 2014 Mar;37(3):187-205. doi: 10.530/ijao.5000307. Epub 2014 Mar 31.
9
Therapeutic potential of mesenchymal stem cells in regenerative medicine.间充质干细胞在再生医学中的治疗潜力。
Stem Cells Int. 2013;2013:496218. doi: 10.1155/2013/496218. Epub 2013 Mar 19.
10
Human Wharton's jelly mesenchymal stem cells maintain the expression of key immunomodulatory molecules when subjected to osteogenic, adipogenic and chondrogenic differentiation in vitro: new perspectives for cellular therapy.人牙髓间充质干细胞在体外成骨、成脂和成软骨分化过程中保持关键免疫调节分子的表达：细胞治疗的新视角。
Curr Stem Cell Res Ther. 2013 Jan;8(1):100-13. doi: 10.2174/1574888x11308010012.

壳聚糖/透明质酸多层膜是用于脐带来源干细胞的生物相容性基质。

Chitosan/hyaluronic acid multilayer films are biocompatible substrate for Wharton's jelly derived stem cells.

作者信息

Dennaoui Hana, Chouery Eliane, Rammal Hassan, Abdel-Razzak Ziad, Harmouch Chaza

机构信息

Laboratory of Applied Biotechnology: Biomolecules, Biotherapies and Bioprocesses, AZM Centre for Biotechnology research and its Applications, Doctoral School of Science and Technology, Lebanese University, Tripoli, Lebanon.

Medical Genetics Unit, Faculty of medicine, Saint Joseph University (USJ), Beirut, Lebanon.

出版信息

Stem Cell Investig. 2018 Dec 20;5:47. doi: 10.21037/sci.2018.12.02. eCollection 2018.

DOI:10.21037/sci.2018.12.02
PMID:30701182
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6327174/
Abstract

BACKGROUND

Discovery of mesenchymal stem cells (MSCs) in various adult human tissues opened the way to new therapeutic strategies involving tissue engineering from these cells. More recently, vascular substitutes have opened the era of vascular engineering by making replacement vessels from purely biological material. The objective of our study was to create a vascular substitute from MSCs using a multilayer polyelectrolyte film based on natural polymers (Chitosan and Hyaluronic Acid).

METHODS

Biocompatibility and cellular behavior were evaluated in this study using MSCs from the Wharton's jelly (WJ) of human umbilical cords. WJ-MSCs adherence was assessed and cells morphology was investigated by Scanning Electron Microscopy (SEM) and actin visualization (Phalloidin).

RESULTS

The number of WJ-MSCs seeded on the (CHI/HA) films was greater than the number of cells seeded on the collagen, as the spectrophotometric measurement showed a large cell proliferation on (CHI/HA) in comparison with collagen. After adhesion, WJ-MSCs showed a fibroblastic morphology on CHI/HA as for control (collagen I). These results were confirmed by cytoskeleton staining.

CONCLUSIONS

The biocompatibility of WJ-MSCs and (CHI/HA) showed the possibility to combine the use of WJ-MSCs and (CHI/HA) films in vascular tissue engineering.

摘要

背景

在多种成人组织中发现间充质干细胞（MSCs）为涉及利用这些细胞进行组织工程的新治疗策略开辟了道路。最近，血管替代物通过使用纯生物材料制造替代血管开启了血管工程时代。我们研究的目的是使用基于天然聚合物（壳聚糖和透明质酸）的多层聚电解质膜从间充质干细胞创建一种血管替代物。

方法

本研究使用来自人脐带华通氏胶（WJ）的间充质干细胞评估生物相容性和细胞行为。评估了WJ-MSCs的黏附情况，并通过扫描电子显微镜（SEM）和肌动蛋白可视化（鬼笔环肽）研究了细胞形态。

结果

如分光光度测量所示，接种在（CHI/HA）膜上的WJ-MSCs数量多于接种在胶原蛋白上的细胞数量，与胶原蛋白相比，（CHI/HA）上有大量细胞增殖。黏附后，WJ-MSCs在CHI/HA上呈现出与对照（I型胶原蛋白）相同的成纤维细胞形态。这些结果通过细胞骨架染色得到证实。

结论

WJ-MSCs与（CHI/HA）的生物相容性表明在血管组织工程中联合使用WJ-MSCs和（CHI/HA）膜具有可能性。