Rammal Hassan, Harmouch Chaza, Maerten Clément, Gaucher Caroline, Boulmedais Fouzia, Schaaf Pierre, Voegel Jean Claude, Laurent-Maquin Dominique, Menu Patrick, Kerdjoudj Halima
UMR 7365, Centre National de la Recherche Scientifique, Université de Lorraine, Biopôle, Faculté de Médecine, 9 avenue de la forêt de Haye, Vandœuvre-lès-Nancy, 54505, France.
Equipe d'Accueil 4691 Biomatériaux et Inflammation en Site Osseux, UFR Odontologie, SFR-CAP Santé (FED 4231), Université de Reims Champagne Ardenne, 1 Avenue du Maréchal Juin, Reims, 51100, France.
J Biomed Mater Res A. 2017 Jan;105(1):292-300. doi: 10.1002/jbm.a.35868. Epub 2016 Oct 31.
Designing convenient substrates is a pertinent parameter that can guide stem cell differentiation. Current research is directed toward differentiating mesenchymal stem cells (MSCs) into endothelial cells (ECs). It is generally accepted that MSCs cannot be easily differentiated into ECs without high concentrations of proangiogenic factors. To guide either bone marrow-derived mesenchymal stem cells (BM-MSCs) and Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) into ECs-like phenotype, poly(allylamine-hydrochloride)/poly(styrene-sulfonate) multilayers film (PAH/PSS) was used as culture coating and compared to type I collagen (as control coating). After 2 weeks of culture and in absence of angiogenic growth factors, PAH/PSS upregulated KDR, PECAM-1, and CDH5 genes, whereas combining PAH/PSS with endothelial growth media (EGM-2 ) led to the production of respective proteins by WJ-MSCs. In contrast, not fully EC-like phenotype is obtained from the differentiation of BM- or WJ-MSCs cultured on type I collagen. Thus, using PAH/PSS coating in synergy with EGM-2 appears as an ideal condition promoting WJ-MSCs differentiation into ECs-like. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 292-300, 2017.
设计便捷的基质是一个可指导干细胞分化的相关参数。当前的研究旨在将间充质干细胞（MSCs）分化为内皮细胞（ECs）。普遍认为，若无高浓度的促血管生成因子，MSCs很难分化为ECs。为了将骨髓来源的间充质干细胞（BM-MSCs）和脐带来源的间充质干细胞（WJ-MSCs）诱导为类ECs表型，使用聚（烯丙胺盐酸盐）/聚（苯乙烯磺酸盐）多层膜（PAH/PSS）作为培养涂层，并与I型胶原（作为对照涂层）进行比较。在无血管生成生长因子的情况下培养2周后，PAH/PSS上调了KDR、PECAM-1和CDH5基因，而将PAH/PSS与内皮生长培养基（EGM-2）结合使用，可使WJ-MSCs产生相应的蛋白质。相比之下，在I型胶原上培养的BM-MSCs或WJ-MSCs分化后未获得完全类ECs表型。因此，将PAH/PSS涂层与EGM-2协同使用似乎是促进WJ-MSCs分化为类ECs的理想条件。© 2016威利期刊公司。《生物医学材料研究杂志》A部分：第105A卷：292 - 300页，2017年。
