1International Ocular Surface Research Center, Institute of Ophthalmology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Jinan University, Guangzhou, China.
2Henan Eye Institute, Henan Eye Hospital, Henan Provincial People's Hospital, Henan University People's Hospital, Zhengzhou, China.
Commun Biol. 2019 Jan 24;2:33. doi: 10.1038/s42003-018-0270-9. eCollection 2019.
Exposure to tobacco smoke is a major public health concern that can also affect ophthalmic health. Based on previous work demonstrating the important role of the sympathetic nervous system (SNS) in corneal wound repair, we postulated that acute tobacco smoke exposure (ATSE) may act through the SNS in the impairment of corneal wound repair. Here we find that ATSE rapidly increases the markers of inflammatory response in normal corneal limbi. After an abrasion injury, ATSE exaggerates inflammation, impairs wound repair, and enhances the expression of nuclear factor-κB (NF-κB) and inflammatory molecules such as interleukin-6 (IL-6) and IL-17. We find that chemical SNS sympathectomy, local adrenergic receptor antagonism, inactivation, and IL-6/IL-17A neutralization can all independently attenuate ATSE-induced excessive inflammatory responses and alleviate their impairment of the healing process. These findings highlight that the SNS may represent a major molecular sensor and mediator of ATSE-induced inflammation.
暴露于烟草烟雾是一个主要的公共卫生关注点，也会影响眼部健康。基于先前的研究表明，交感神经系统（SNS）在角膜伤口修复中起着重要作用，我们推测急性烟草烟雾暴露（ATSE）可能通过 SNS 作用于角膜伤口修复的损伤。在这里，我们发现 ATSE 可迅速增加正常角膜缘的炎症反应标志物。在刮伤损伤后，ATSE 加剧了炎症反应，损害了伤口修复，并增强了核因子-κB（NF-κB）和炎症分子（如白细胞介素-6（IL-6）和白细胞介素-17（IL-17））的表达。我们发现化学性 SNS 交感神经切除术、局部肾上腺素能受体拮抗作用、失活以及 IL-6/IL-17A 中和均可独立减轻 ATSE 诱导的过度炎症反应，并缓解其对愈合过程的损害。这些发现强调，SNS 可能是 ATSE 诱导的炎症的主要分子传感器和介质。
