Armenta-Medina Alma, Gillmor C Stewart
Laboratorio Nacional de Genómica para la Biodiversidad (Langebio), Unidad de Genómica Avanzada, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, Guanajuato, Mexico.
Methods Mol Biol. 2019;1932:1-14. doi: 10.1007/978-1-4939-9042-9_1.
MicroRNAs play important roles in posttranscriptional regulation of plant development, metabolism, and abiotic stress responses. The recent generation of massive amounts of small RNA sequence data, along with development of bioinformatic tools to identify miRNAs and their mRNA targets, has led to an explosion of newly identified putative miRNAs in plants. Genome editing techniques like CRISPR-Cas9 will allow us to study the biological role of these potential novel miRNAs by efficiently targeting both the miRNA and its mRNA target. In this chapter, we review bioinformatic tools and experimental methods for the identification and functional characterization of miRNAs and their target mRNAs in plants.
微小RNA在植物发育、代谢及非生物胁迫响应的转录后调控中发挥着重要作用。近期大量小RNA序列数据的产生，以及用于鉴定微小RNA及其mRNA靶标的生物信息学工具的发展，导致植物中新鉴定的假定微小RNA数量激增。像CRISPR-Cas9这样的基因组编辑技术将使我们能够通过有效靶向微小RNA及其mRNA靶标来研究这些潜在新微小RNA的生物学作用。在本章中，我们综述了用于鉴定植物微小RNA及其靶标mRNA并对其进行功能表征的生物信息学工具和实验方法。
