Department of chemistry, Simon Fraser University, Burnaby, British Columbia, Canada.
Department of chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Electrophoresis. 2019 May;40(10):1478-1485. doi: 10.1002/elps.201800451. Epub 2019 Feb 5.
Here, we report a microfluidic same-single-cell analysis to study the inhibition of multidrug resistance due to drug efflux on single leukemic cells. Drug efflux inhibition was investigated in the microfluidic chip using two different fluorescence detection systems, namely, a compact single-cell bioanalyzer and the conventional optical detection system constructed from an inverted microscope and a microphotometer. More importantly, a compact signal generator was used to conduct dielectrophoretic cell trapping together with the compact SCB. By using the DEP force, a single acute myeloid leukemia cell was trapped in the cell retention structure of the chip. This allowed us to detect dye accumulation in the MDR leukemic cells in the presence of cyclosporine A (CsA). CsA and rhodamine 123 were used as the P-glycoprotein inhibitor and fluorescent dye, respectively. The result showed that the Rh123 fluorescence signal in a single-cell increased dramatically over its same-cell control on both fluorescence detection systems due to the inhibition by CsA.
在这里，我们报告了一种微流控同一样单细胞分析方法，用于研究由于药物外排而对单个白血病细胞的多药耐药性的抑制作用。使用两种不同的荧光检测系统，即紧凑型单细胞生物分析仪和由倒置显微镜和微光度计构建的传统光学检测系统，在微流控芯片中研究了药物外排抑制作用。更重要的是，使用紧凑型信号发生器与紧凑型 SCB 一起进行介电泳细胞捕获。通过使用 DEP 力，将单个急性髓系白血病细胞捕获在芯片的细胞保留结构中。这使我们能够在环孢菌素 A (CsA) 的存在下检测到多药耐药性白血病细胞中的染料积累。CsA 和罗丹明 123 分别用作 P-糖蛋白抑制剂和荧光染料。结果表明，由于 CsA 的抑制作用，单个细胞中的 Rh123 荧光信号在两种荧光检测系统上均比相同细胞对照显著增加。
