Northeastern University, Sino-Dutch Biomedical and Information Engineering School, Shenyang, China.
Chinese Academy of Sciences, Institute of Automation, CAS Key Laboratory of Molecular Imaging, Beiji, China.
J Biomed Opt. 2019 Jan;24(1):1-8. doi: 10.1117/1.JBO.24.1.016003.
The objective of our study is to develop a multimodality approach by combining magnetic resonance imaging (MRI) and optical imaging methods to assess acute murine colitis at the macro- and microscopic level. In vivo MRI is used to measure the cross-sectional areas of colons at the macroscopic level. Dual-color confocal laser endomicroscopy (CLE) allows in vivo examination of the fluorescently labeled epithelial cells and microvessels in the mucosa with a spatial resolution of ∼1.4 μm during ongoing endoscopy. To further validate the structural changes of the colons in three-dimensions, ex vivo light-sheet fluorescence microscopy (LSFM) is applied for in-toto imaging of cleared colon sections. MRI, LSFM, and CLE findings are significantly correlated with histological scoring (p < 0.01) and the inflammation-associated activity index (p < 0.01). Our multimodality imaging technique permits visualization of mucosa in colitis at different scales, which can enhance our understanding of the pathogenesis of inflammatory bowel diseases.
我们的研究目的是通过结合磁共振成像（MRI）和光学成像方法来开发一种多模态方法，以在宏观和微观水平上评估急性鼠结肠炎。体内 MRI 用于测量宏观水平上结肠的横截面积。双色共聚焦激光内窥镜检查术（CLE）允许在进行内窥镜检查的同时，以约 1.4 μm 的空间分辨率对荧光标记的上皮细胞和粘膜中的微血管进行体内检查。为了进一步验证结肠在三维结构上的变化，应用离体光片荧光显微镜（LSFM）对清除的结肠切片进行全内成像。MRI、LSFM 和 CLE 的发现与组织学评分（p < 0.01）和炎症相关的活动指数（p < 0.01）显著相关。我们的多模态成像技术允许在不同的尺度上可视化结肠炎中的粘膜，可以增强我们对炎症性肠病发病机制的理解。
