Ninnemann J L
J Immunol. 1978 May;120(5):1573-9.
Using normal human lymphocytes isolated by sedimentation and cotton column adherence, we have developed a reliable assay of immunosuppression of PHA-induced blastogenesis by serum from selected melanoma patients. These lymphocyte cultures contained both responder cells (subpopulation x) and regulator cells (subpopulation y). Lymphocytes isolated by gradient centrifugation on sodium metrizoate-Ficoll contained responder cells (x) but no regulator cells (y). Cultures of lymphocytes isolated by this method were stimulated by PHA but were not suppressed by the addition of melanoma serum. When lymphocytes were isolated by a cotton column adherence/Lymphoprep centrifugation-double separation, subpopulations (x) and (y) were isolated. We have established that both subpopulations are necessary for suppression to occur, and that (y) operates as the regulator of (x). Finally, by manipulating B cell and T cell populations isolated by nylon column adherence or AET rosette separation, we have determined that the regulator ability of subpopulation (y) is the result of B cell activation of suppressor T cells.
利用通过沉降和棉柱黏附分离出的正常人淋巴细胞，我们开发出一种可靠的检测方法，用于检测特定黑色素瘤患者血清对PHA诱导的淋巴细胞增殖的免疫抑制作用。这些淋巴细胞培养物中既含有反应细胞（亚群x），也含有调节细胞（亚群y）。通过在甲泛影酸钠-菲可溶液上进行梯度离心分离出的淋巴细胞含有反应细胞（x），但不含调节细胞（y）。用这种方法分离出的淋巴细胞培养物受到PHA刺激，但添加黑色素瘤血清后不会受到抑制。当通过棉柱黏附/淋巴细胞分离液离心双重分离法分离淋巴细胞时，亚群（x）和（y）被分离出来。我们已经确定，两个亚群对于抑制作用的发生都是必需的，并且（y）作为（x）的调节因子发挥作用。最后，通过操控通过尼龙柱黏附或AET花环分离法分离出的B细胞和T细胞群体，我们确定亚群（y）的调节能力是抑制性T细胞的B细胞活化的结果。
