Sakane T, Green I
J Immunol. 1977 Sep;119(3):1169-78.
Human peripheral blood lymphocytes were stimulated by concanavalin A (Con A) and then evaluated by their suppressive activity for thymus-derived (T) cell- and bone marrow-derived (B) cell-proliferative responses to mitogen and allogeneic cells. Con A-activated T cells markedly suppressed these responses, but Con A-activated B cells failed to demonstrate suppressor activity. Discontinuous bovine serum albumin (BSA) density gradient separation of T cells which had been activated by Con A demonstrated that a fraction containing blast cells as well as fractions containing unproliferated cells manifest the same degree of suppressor capabilities. However, when density gradient separation of T cells followed by subsequent incubation with Con A was performed, fractions of proliferating cells of low density exhibited no suppression; a fraction containing high density T cells produced marked suppression, but this fraction incorporated only little thymidine in response to Con A. Thus, these studies indicate that Con A-induced suppressor T cells belong to a distinctive subpopulation which has already been programmed to express this function before exposure to Con A and that cell proliferation may not be a prerequisite for the development of such suppressor T cells.
用人外周血淋巴细胞经刀豆蛋白A（Con A）刺激，然后通过其对胸腺来源（T）细胞和骨髓来源（B）细胞对有丝分裂原和同种异体细胞增殖反应的抑制活性进行评估。Con A激活的T细胞显著抑制这些反应，但Con A激活的B细胞未表现出抑制活性。对经Con A激活的T细胞进行不连续牛血清白蛋白（BSA）密度梯度分离表明，含有母细胞的部分以及含有未增殖细胞的部分表现出相同程度的抑制能力。然而，当对T细胞进行密度梯度分离并随后与Con A孵育时，低密度增殖细胞部分未表现出抑制作用；含有高密度T细胞的部分产生显著抑制作用，但该部分对Con A的反应中仅掺入少量胸苷。因此，这些研究表明，Con A诱导的抑制性T细胞属于一个独特的亚群，该亚群在接触Con A之前就已被编程表达这种功能，并且细胞增殖可能不是此类抑制性T细胞发育的先决条件。
