Domingues Rodrigo R, Garrone-Neto Domingos, Hilsdorf Alexandre W S, Gadig Otto B F
Departamento de Ciências do Mar, UNIFESP - Universidade Federal de São Paulo, Instituto do Mar, São Paulo, Brazil.
UNESP - Universidade Estadual Paulista, Campus Experimental de Registro, São Paulo, Brazil.
J Fish Biol. 2019 Mar;94(3):512-516. doi: 10.1111/jfb.13919. Epub 2019 Feb 22.
In this study we tested the use of mucus from five species of Neotropical marine batoid elasmobranchs to extract genomic DNA for barcoding and phylogenetic analysis. The DNA from all individuals sampled was successfully amplified and sequenced for molecular barcode, allowing 99-100% accuracy to the species level. This method proved to provide reliable and good-quality DNA for barcoding and phylogenetic analysis of Neotropical elasmobranchs, through rapid handling and with low disturbance to animals.
在本研究中，我们测试了利用五种新热带区海洋魟类板鳃亚纲鱼类的黏液来提取基因组DNA，用于条形码编码和系统发育分析。对所有采样个体的DNA进行了成功扩增，并对分子条形码进行了测序，在物种水平上的准确率达到了99%-100%。该方法经证明能够通过快速处理且对动物干扰较小，为新热带区板鳃亚纲鱼类的条形码编码和系统发育分析提供可靠且高质量的DNA。
