Kagerbauer Simone Maria, Kadera Vojtech, Gordan Lucia Maria, Blobner Manfred, Török Elisabeth, Schmid Sebastian, Podtschaske Armin Horst, Jungwirth Bettina
Technical University of Munich, School of Medicine, Klinikum rechts der Isar, Department of Anaesthesiology and Intensive Care Medicine, Ismaninger Str. 22, 81675 München, Germany.
Technical University of Munich, School of Medicine, Klinikum rechts der Isar, Department of Anaesthesiology and Intensive Care Medicine, Ismaninger Str. 22, 81675 München, Germany.
Behav Brain Res. 2019 May 2;363:13-22. doi: 10.1016/j.bbr.2019.01.050. Epub 2019 Jan 28.
The aim of this study was to detect differences in functional outcome after experimental subarachnoid haemorrhage (SAH) in rodents with different hormonal status. For this purpose, the endovascular perforation model was applied to four groups of Sprague-Dawley-Rats: male intact, male neutered, female intact and female neutered animals. Initial impact was measured by ICP, CPP and cerebral blood flow in the first hour after SAH. From day 4-14, the modified hole board test was applied to assess functional and neuro-cognitive outcome. Histological outcome was examined in the motor cortex and hippocampus of each hemisphere. Mortality was highest in the female intact group albeit not statistically significant. Physiologic parameters did not differ significantly between groups either. In the modified hole board test, male intact animals showed a greater impairment of declarative memory than the female intact and neutered groups. However, male intact animals showed greater avoidance behaviour and male animals revealed higher anxiety levels independent of hormonal status. No differences in histological damage of hippocampus and motor cortex between groups could be shown. We therefore speculate that the marginal deficits in cognitive performance that are shown by the male intact group in the modified hole board test are mostly caused by higher anxiety levels and cannot be interpreted as pure cognitive impairment.
本研究的目的是检测不同激素状态的啮齿动物在实验性蛛网膜下腔出血（SAH）后功能结局的差异。为此，将血管内穿刺模型应用于四组Sprague-Dawley大鼠：雄性未阉割、雄性阉割、雌性未阉割和雌性阉割动物。在SAH后的第一小时，通过颅内压（ICP）、脑灌注压（CPP）和脑血流量来测量初始影响。从第4天到第14天，应用改良的洞板试验来评估功能和神经认知结局。在每个半球的运动皮层和海马体中检查组织学结局。雌性未阉割组的死亡率最高，尽管无统计学意义。各组之间的生理参数也无显著差异。在改良的洞板试验中，雄性未阉割动物的陈述性记忆损伤比雌性未阉割和阉割组更大。然而，雄性未阉割动物表现出更强的回避行为，并且雄性动物无论激素状态如何都表现出更高的焦虑水平。各组之间海马体和运动皮层的组织学损伤无差异。因此，我们推测雄性未阉割组在改良洞板试验中表现出的认知能力的轻微缺陷主要是由较高的焦虑水平引起的，不能被解释为单纯的认知障碍。
