The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu, 214122, China.
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu, 214122, China.
J Biotechnol. 2019 Mar 10;293:66-71. doi: 10.1016/j.jbiotec.2019.01.012. Epub 2019 Jan 28.
Enzymatic degradation of urea, the precursor of carcinogenic compound ethylcarbamate in rice wine, is always attractive. In the present study, we achieved high efficient production of Bacillus paralicheniformis iron-containing urease (Bp_Urease) in B. subtilis with the food-grade expression system. After reassembly of the urease gene cluster with inserting ribosome binding site (RBS), the production was increased from 38 U/L to 187 U/L. After altering the position of ureC and co-expressing the iron transporter encoding gene ureH, the activity was further increased to 1307 U/L. Eventually, the urease production was improved to 21,964 U/L in 3-L fermentor, which is the highest reported value to date. Food-grade production of the iron-containing urease would be favorable to the practical applications in food industries.
米酒中致癌化合物氨基甲酸乙酯的前体尿素的酶促降解一直很有吸引力。在本研究中，我们在枯草芽孢杆菌中利用食品级表达系统实现了产碱假单胞菌含铁脲酶（Bp_Urease）的高效生产。通过重新组装脲酶基因簇并插入核糖体结合位点（RBS），产量从 38 U/L 增加到 187 U/L。改变 ureC 的位置并共表达铁转运蛋白编码基因 ureH 后，活性进一步提高到 1307 U/L。最终，在 3-L 发酵罐中脲酶的产量提高到 21964 U/L，这是迄今为止报道的最高值。食品级含铁脲酶的生产有利于在食品工业中的实际应用。
