Love G L, Restrepo C
Department of Pathology, Louisiana State University Medical Center, New Orleans.
Mod Pathol. 1988 Jul;1(4):256-61.
The histogenesis of the Aschoff body of rheumatic carditis is controversial. Proliferative Aschoff bodies in heart sections from 6 patients with acute rheumatic heart disease were tested by avidin-biotin peroxidase labeling methods for the presence of desmin, muscle-specific actin, S-100, neurofilament, leukocyte common antigen, receptor for Ulex europeus I lectin, Factor VIII-related antigen, vimentin, alpha 1-antichymotrypsin, and myeloid/histiocyte antigen. Lack of Aschoff body labeling for desmin and muscle-specific actin, S-100 and neurofilament, and Ulex europeus I and Factor VIII-related antigen is not consistent with histogenesis from smooth or striated cardiac muscle, nerve or nerve sheath, and lymphatic or vascular endothelium, respectively. Strong labeling of Aschoff body cells for vimentin is evidence for a mesenchymal origin, and labeling for myeloid/histiocyte antigen is consistent with a histiocytic origin. Furthermore, weak, variable labeling of Aschoff body cells for leukocyte common antigen suggests that at least some Aschoff body cells were originally derived from blood-borne monocytes. Both multinucleated Aschoff cells and "owl's eye," Anitschkow cells label identically, suggesting a common origin. Alpha 1-Antichymotrypsin, a widely utilized marker of histiocytes, was unexpectedly negative. Perhaps histiocytes that form Aschoff bodies do not express this lysosomal enzyme. Aschoff bodies appear to be a unique and distinctive form of granuloma.
风湿性心肌炎阿绍夫小体的组织发生存在争议。采用抗生物素蛋白-生物素过氧化物酶标记法，对6例急性风湿性心脏病患者心脏切片中的增殖性阿绍夫小体进行检测，以确定是否存在结蛋白、肌肉特异性肌动蛋白、S-100、神经丝、白细胞共同抗原、欧洲荆豆I凝集素受体、VIII因子相关抗原、波形蛋白、α1抗糜蛋白酶和髓样/组织细胞抗原。阿绍夫小体对结蛋白和肌肉特异性肌动蛋白、S-100和神经丝、欧洲荆豆I和VIII因子相关抗原均无标记，这分别与平滑肌或横纹肌、神经或神经鞘、淋巴管或血管内皮的组织发生不一致。阿绍夫小体细胞对波形蛋白有强标记，证明其起源于间充质，对髓样/组织细胞抗原的标记与组织细胞起源一致。此外，阿绍夫小体细胞对白细胞共同抗原的标记较弱且变化不定，提示至少部分阿绍夫小体细胞最初来源于血源性单核细胞。多核阿绍夫细胞和“枭眼”样阿尼奇科夫细胞的标记相同，提示它们有共同起源。α1抗糜蛋白酶是一种广泛应用的组织细胞标志物，结果却意外呈阴性。或许形成阿绍夫小体的组织细胞不表达这种溶酶体酶。阿绍夫小体似乎是一种独特且有别于其他的肉芽肿形式。
