Kleveland G, Egeland T, Lea T
Institute of Immunology and Rheumatology, Rikshospitalet University Hospital, Oslo, Norway.
Scand J Rheumatol Suppl. 1988;75:15-24. doi: 10.3109/03009748809096734.
An enzyme-linked immuno-sorbent assay (ELISA) for quantitation of rheumatoid factors (RF) of IgM, IgA and IgG isotypes has been established. A complex of human serum albumin (HSA) and rabbit IgG anti-HSA antibodies is used as antigen for RF. The binding of RF is detected by stepwise additions of biotinylated monoclonal antibodies specific for human IgM, IgG or IgA, alkaline phosphatase-conjugated streptavidin, and substrate. The assay is simple and applicable to routine testing of large numbers of sera. It discriminates between false and true IgG-RF by papain digestion of sera that turn out positive by the screening for IgG-RF. Of 241 randomly selected patients with rheumatoid arthritis (RA) as well as other rheumatoid and infectious diseases, 110 were Waaler-Rose-positive while 127 were IgM-RF-positive in ELISA. The correlation between the Waaler-Rose test and IgM-RF ELISA was highly significant (r = 0.82). By testing 65 of these sera (all IgM-RF positive), 25 (39%) were also true IgG-RF positive (42 (64%) in the screening). When 40 Waaler-Rose-positive RA patients were tested, 20 and 21 were also positive for IgG- and IgA-RF, respectively. Eight IgM-, one IgA- and no IgG-RF positive tests were recovered when 48 Waaler-Rose negative RA patients were studied.
已建立一种用于定量检测IgM、IgA和IgG同种型类风湿因子（RF）的酶联免疫吸附测定（ELISA）。人血清白蛋白（HSA）与兔抗HSA IgG抗体的复合物用作RF的抗原。通过逐步添加对人IgM、IgG或IgA特异的生物素化单克隆抗体、碱性磷酸酶偶联的链霉亲和素和底物来检测RF的结合。该测定方法简单，适用于大量血清的常规检测。通过对经IgG-RF筛查呈阳性的血清进行木瓜蛋白酶消化，可区分假阳性和真阳性IgG-RF。在随机选择的241例类风湿关节炎（RA）以及其他类风湿和感染性疾病患者中，110例Waaler-Rose试验呈阳性，而ELISA检测中127例IgM-RF呈阳性。Waaler-Rose试验与IgM-RF ELISA之间的相关性非常显著（r = 0.82）。对其中65份血清（均为IgM-RF阳性）进行检测，25份（39%）同时也是真阳性IgG-RF（筛查中为42份（64%））。对40例Waaler-Rose试验阳性的RA患者进行检测时，分别有20例和21例IgG-RF和IgA-RF也呈阳性。对48例Waaler-Rose试验阴性的RA患者进行研究时，发现8例IgM-RF、1例IgA-RF阳性，未发现IgG-RF阳性检测结果。
