Sensors and Electron Devices Directorate, U.S. Army Research Laboratory, 2800 Powder Mill Rd, Adelphi, MD, United States.
Sensors and Electron Devices Directorate, U.S. Army Research Laboratory, 2800 Powder Mill Rd, Adelphi, MD, United States.
Methods. 2019 Apr 1;158:12-16. doi: 10.1016/j.ymeth.2019.01.014. Epub 2019 Jan 30.
The need for the functionalization of magnetic, water-soluble dyed microspheres with peptides is apparent with the ever-growing biointeraction capabilities and the increased use of dyed microspheres in multiplex, microsphere-based detection assays. This method describes the attachment of any peptide to dyed magnetic microspheres regardless of peptide length, size, or sequence. The method exploits 'click' chemistry with short reaction times in a mixed organic/water system for simultaneous selective surface functionalization and reduction of microsphere dye leaching. All optimization studies were performed using a Luminex 200 assay platform, but the functionalized microspheres are capable of use in any similar multiplex format.
具有生物相互作用能力的不断增强，以及染色微球在多重、基于微球的检测分析中的应用日益广泛，因此需要对磁性、水溶性染色微球进行功能化，使其与肽结合。本方法描述了将任何肽连接到染色磁性微球上，而与肽的长度、大小或序列无关。该方法利用混合有机/水体系中的“点击”化学，具有较短的反应时间，可同时进行选择性表面功能化和减少微球染料浸出。所有优化研究均使用 Luminex 200 分析平台进行，但功能化的微球可用于任何类似的多重格式。
