School of Integrated Chinese and Western Medicine, Binzhou Medical University, Yantai, Shangdong 264003, PR China; Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research, Huaiyin Institute of Technology, Huai'an 223003, Jiangsu, PR China.
Shandong College of Traditional Chinese Medicine, Yantai, Shangdong 264199, PR China.
Int J Biol Macromol. 2019 May 1;128:804-813. doi: 10.1016/j.ijbiomac.2019.01.193. Epub 2019 Jan 30.
The purpose of this paper was to investigate the effects and mechanism of polysaccharide (PAOF) from Alpiniae oxyphyllae fructus on urinary incontinence (UI) in old-age hydruric model rats (OHMR). Results suggested that PAOF can significantly reduce the urination volume, Na, Cl emission and increase K excretion of OHMR. In addition, PAOF can increase the content of aldosterone (ALD) and antidiuretic hormone (ADH) in blood of OHMR. The coefficients of spleen, thymus and adrenal of OHMR were improved by PAOF. Furthermore, PAOF can not only elevate significantly the expression of β3-adrenoceptor mRNA in bladder detrusor of OHMR, but also increase the content of adenylate cyclase (AC), cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) in bladder detrusor of OHMR. Meanwhile, PAOF can elevate significantly the expression of PKA protein in bladder detrusor of rats with polyuria. The data implied that PAOF may offer therapeutic potential against UI.
本研究旨在探讨益智果多糖（PAOF）对老年高尿酸模型大鼠（OHMR）尿失禁（UI）的作用及其机制。结果表明，PAOF 能显著减少 OHMR 的排尿量、Na+、Cl-排泄量，并增加 K+排泄量。此外，PAOF 能增加 OHMR 血液中醛固酮（ALD）和抗利尿激素（ADH）的含量。PAOF 还能改善 OHMR 的脾、胸腺和肾上腺系数。此外，PAOF 不仅能显著提高 OHMR 膀胱逼尿肌中β3-肾上腺素能受体 mRNA 的表达，还能增加膀胱逼尿肌中环磷酸腺苷（cAMP）和蛋白激酶 A（PKA）的含量。同时，PAOF 能显著提高多尿大鼠膀胱逼尿肌中 PKA 蛋白的表达。这些数据表明，PAOF 可能具有治疗 UI 的潜力。
