Miranda J C, Cavicchia J C
Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.
Tissue Cell. 1988;20(4):611-9. doi: 10.1016/0040-8166(88)90062-6.
Intercellular junctions in the seminiferous epithelium of the testis of Triatoma infestans were examined by conventional electron microscopy, tannic acid fixation, electron-opaque tracers, and freeze-fracture techniques. Distinctive aspects of the intercellular junctions are described in different regions of the testis follicles. In the basal region, close to the basal lamina, smooth septate junctions intermingled with gap junctions were observed between sustentacular cells. In the parabasal regions of the seminiferous epithelium, plated septate junctions, 'molluscous and arthropod' type (according to the classification of Green, 1981), were observed. Over the above junctions, in the central regions, also located between sustentacular cell membranes, parallel rows of intramembrane particles form successive belts of widely spaced septate junctions in an atypical configuration. Invertebrate gap junctions were also observed between adjacent spermatocyte membranes.
采用传统电子显微镜、单宁酸固定、电子不透明示踪剂和冷冻断裂技术，对骚扰锥蝽睾丸生精上皮中的细胞间连接进行了检查。在睾丸滤泡的不同区域描述了细胞间连接的独特方面。在靠近基膜的基部区域，支持细胞之间观察到平滑的分隔连接与间隙连接混合存在。在生精上皮的副基部区域，观察到板状分隔连接，即“软体动物和节肢动物”类型（根据格林1981年的分类）。在上述连接之上，在中央区域，也位于支持细胞膜之间，平行排列的膜内颗粒形成了具有非典型构型的宽间距分隔连接的连续带。在相邻的精母细胞膜之间也观察到了无脊椎动物间隙连接。
