Connell C J
J Cell Biol. 1978 Jan;76(1):57-75. doi: 10.1083/jcb.76.1.57.
What appear to be true septate junctions by all techniques currently available for the cytological identification of intercellular junctions are part of a complex junction that interconnects the Sertoli cells of the canine testis. In the seminiferous epithelium, septate junctions are located basal to belts of tight junctions. In thin sections, septate junctions appear as double, parallel, transverse connections or septa spanning an approximately 90-A intercellular space between adjacent Sertoli cells. In en face sections of lanthanum-aldehyde-perfused specimens, the septa themselves exclude lanthanum and appear as electron-lucent lines arranged in a series of double, parallel rows on a background of electron-dense lanthanum. In freeze-fracture replicas this vertebrate septate junction appears as double, parallel rows of individual or fused particles which conform to the distribution of the intercellular septa. Septate junctions can be clearly distinguished from tight junctions as tight junctions prevent the movement of lanthanum tracer toward the lumen, appear as single rows of individual or fused particles in interlacing patterns within freeze-fracture replicas, and are seen as areas of close membrane apposition in thin sections. Both the septate junction and the tight junction are associated with specializations of the Sertoli cell cytoplasm. This is the first demonstration in a vertebrate tissue of a true septate junction.
目前所有用于细胞间连接的细胞学鉴定技术所显示的看似真正的分隔连接，是连接犬睾丸支持细胞的复杂连接的一部分。在生精上皮中，分隔连接位于紧密连接带的基部。在薄切片中，分隔连接表现为跨越相邻支持细胞间约90埃细胞间隙的双平行横向连接或隔膜。在经镧 - 醛灌注标本的表面切片中，隔膜本身排斥镧，表现为在电子致密镧背景上呈双平行排排列的电子透明线。在冷冻蚀刻复制品中，这种脊椎动物的分隔连接表现为单个或融合颗粒的双平行排，与细胞间隔膜的分布一致。分隔连接可与紧密连接明显区分，因为紧密连接可阻止镧示踪剂向管腔移动，在冷冻蚀刻复制品中表现为交错模式的单个或融合颗粒的单排，在薄切片中表现为紧密的膜贴合区域。分隔连接和紧密连接都与支持细胞细胞质的特化有关。这是在脊椎动物组织中首次证明真正的分隔连接。
