Horie T, Ohmori C, Koyama S, Saitoh O, Mutoh T, Okuma A, Okayasu M
First Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Tohoku J Exp Med. 1988 Oct;156(2):109-20. doi: 10.1620/tjem.156.109.
We have investigated the relationship between acute airway hyperresponsiveness and polymorphonuclear cell (PMN) migration in airways following saline or endotoxin (ET) aerosol inhalation in 63 guinea pigs. In 20 of these animals, granulocytopenia was induced by prior treatment with hydroxyurea (HU). Airway responsiveness (AR) to inhaled methacholine, together with leukocyte counts in bronchoalveolar lavage fluid (BALF) and in tracheal epithelium, was also examined before and at 30 min, 1, 3 and 6 hr after saline or ET inhalation. In saline inhalation groups, AR and PMN counts in BALF and in tracheal epithelium did not differ from control animals at any time points. However, in ET inhalation groups AR increased significantly at 1 and 3 hr and returned to the pre-exposure level at 6 hr. This period of hyperresponsiveness was associated with an increment of PMN migration into tracheal epithelium. However, the increment of PMN counts in BALF was delayed to 3 and 6 hr after ET. In HU treated animals, AR and PMN counts in BALF and in tracheal epithelium did not change during 6 hr after ET inhalation. These results suggest that the hyperresponsiveness induced by ET inhalation either depends upon PMN migration into the tracheal epithelium.
我们研究了63只豚鼠在吸入盐水或内毒素（ET）气雾剂后，急性气道高反应性与气道中多形核细胞（PMN）迁移之间的关系。在其中20只动物中，通过预先用羟基脲（HU）处理诱导粒细胞减少。在吸入盐水或ET之前以及之后30分钟、1小时、3小时和6小时，还检测了对吸入乙酰甲胆碱的气道反应性（AR），以及支气管肺泡灌洗液（BALF）和气管上皮中的白细胞计数。在吸入盐水组中，BALF和气管上皮中的AR和PMN计数在任何时间点均与对照动物无差异。然而，在吸入ET组中，AR在1小时和3小时时显著增加，并在6小时时恢复到暴露前水平。这段高反应期与PMN向气管上皮的迁移增加有关。然而，BALF中PMN计数的增加在ET后延迟至3小时和6小时。在HU处理的动物中，吸入ET后6小时内，BALF和气管上皮中的AR和PMN计数没有变化。这些结果表明，吸入ET诱导的高反应性要么取决于PMN向气管上皮的迁移。
