Department of Chemistry and Polymer Science, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa.
Department of Biochemistry, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa.
Molecules. 2019 Jan 31;24(3):508. doi: 10.3390/molecules24030508.
Two commercially available enzymes, Dextrozyme (α-amylase) and Esperase (protease), were covalently immobilized on non-woven electrospun poly(styrene--maleic anhydride) nanofiber mats with partial retention of their catalytic activity. Immobilization was achieved for the enzymes on their own as well as in different combinations with an additional enzyme, β-galactosidase, on the same non-woven nanofiber mat. This experiment yielded a universal method for immobilizing different combinations of enzymes with nanofibrous mats containing maleic anhydride (MAnh) residues in the polymer backbone.
两种市售酶，Dextrozyme（α-淀粉酶）和 Esperase（蛋白酶），通过共价键固定在非织造电纺聚（苯乙烯-马来酸酐）纳米纤维垫上，同时保持部分催化活性。酶可以单独固定，也可以与另一种酶β-半乳糖苷酶组合固定在同一块无纺纳米纤维垫上。该实验提供了一种通用的方法，可以将不同组合的酶与含有马来酸酐（MAnh）残基的纳米纤维垫固定在一起，这些 MAnh 残基位于聚合物主链中。
