短乳杆菌 MF179529 对奶牛源李斯特菌的抑菌效果：体内证据。
Antilisterial efficacy of Lactobacillus brevis MF179529 from cow: an in vivo evidence.
机构信息
Department of Zoology, University of the Punjab , Quaid-i-Azam Campus, Lahore, Pakistan.
Department of Zoology, Government College University, Lahore, Pakistan.
出版信息
BMC Complement Altern Med. 2019 Feb 1;19(1):37. doi: 10.1186/s12906-019-2444-5.
BACKGROUND
Listeria monocytogenes is an opportunistic foodborne pathogen that causes human Listeriosis and high mortality particularly in immunocompromised individuals. Pregnant women are more prone to L. monocytogenes infection resulting in abortions. In the present study, antilisterial activity of Lactobacillus brevis (LB) MF179529, a probiotic bacterial strain, was investigated in a murine model.
METHODS
Initially a pilot study was conducted to determine the dose of L. monocytogenes required to cause symptomatic listeriosis. In the main trial, mice were divided into 4 groups. Group I was kept as negative control, group II was exposed to L. monocytogenes and maintained as positive control. Group III was fed with L. brevis only, while group IV received L. brevis for 3 days prior to L. monocytogenes infection. A volume of 200 μl of L. monocytogenes ATCC 19115 and L. brevis MF179529 bacterial suspension corresponding to cell density of 10CFU/ml were given to respective groups by intragastric route. Progress of infection was monitored for 7 days including general health scoring, listeria dispersion in organs, bacterial load in intestine and blood biochemistry were recorded on 3rd, 5th and 7th days post infection (dpi).
RESULTS
Clinical listeriosis was induced by 10CFU/ml of L. monocytogenes ATCC 19115 in mice. Animals of group IV displayed minor signs of infection. L. brevis supplementation resulted in significant reduction in dispersion and propagation of L. monocytogenes in liver, spleen and intestine. L. brevis MF179529 consumption led to a significant elevation of number of lactic acid bacteria and reduction of total plate count, anaerobic count and coliform population in intestine. Moreover, total leukocyte and neutrophil counts of treated animals were similar to the negative control while positive control group displayed higher number. Safety evaluation of L. brevis was performed by monitoring general health, hematological and serological parameters of L. brevis fed and negative control group (group III and I). No significant difference in feed intake, body temperature, body weight and blood picture could be detected in L. brevis supplemented and control groups.
CONCLUSION
Our results indicate ameliorative role of L. brevis in L. monocytogenes infection and suggest that L. brevis could be used for prophylactic measure.
背景
李斯特菌是一种机会性食源性病原体，可导致人类李斯特菌病，尤其是在免疫功能低下的个体中死亡率很高。孕妇更容易感染李斯特菌，导致流产。在本研究中，研究了益生菌菌株短乳杆菌（LB）MF179529 对小鼠模型的抗李斯特菌活性。
方法
最初进行了一项初步研究，以确定引起有症状李斯特菌病所需的李斯特菌剂量。在主要试验中，将小鼠分为 4 组。第 I 组作为阴性对照，第 II 组暴露于李斯特菌并作为阳性对照。第 III 组仅用短乳杆菌喂养，而第 IV 组在感染李斯特菌前 3 天用短乳杆菌喂养。通过胃内途径向各组给予 200μl 李斯特菌 ATCC 19115 和短乳杆菌 MF179529 细菌悬浮液，细胞密度为 10CFU/ml。在感染后第 3、5 和 7 天记录 7 天的感染进展，包括一般健康评分、器官中李斯特菌的分布、肠道和血液生化中的细菌负荷。
结果
用 10CFU/ml 的李斯特菌 ATCC 19115 在小鼠中诱导临床李斯特菌病。第 IV 组的动物表现出轻微的感染迹象。短乳杆菌的补充导致李斯特菌在肝脏、脾脏和肠道中的分布和繁殖显著减少。短乳杆菌 MF179529 的消耗导致肠道中乳酸菌数量显著增加，总平板计数、厌氧计数和大肠菌群减少。此外，治疗动物的总白细胞和中性粒细胞计数与阴性对照组相似，而阳性对照组的计数较高。通过监测短乳杆菌喂养和阴性对照组（第 III 组和第 I 组）的一般健康、血液学和血清学参数来评估短乳杆菌的安全性。在补充短乳杆菌和对照组中未检测到饲料摄入量、体温、体重和血象的显著差异。
结论
我们的结果表明短乳杆菌在李斯特菌感染中的改善作用，并表明短乳杆菌可用于预防措施。
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