Faradji A, Andreu G, Pillier-Loriette C, Bohbot A, Nicod A, Autran B, Bergerat J P, Rio B, Leblond V, Binet J L
CHU Hautepierre, BMT Unit, Strasbourg, France.
Vox Sang. 1988;55(3):133-8. doi: 10.1111/j.1423-0410.1988.tb05079.x.
In the present study, we report the results of our evaluation of the use of the continuous-flow cell separator Cobe 2997 to isolate from human bone marrow (BM) aspirates the mononuclear cell (MNC) fraction containing hematopoietic stem cells. This MNC concentrate is isolated in 15% of the original BM volume and contains 23% of the initial nucleated cells. It is enriched as concerns the BM MNC fraction (lymphocytes + monocytes recovery; 80%), whereas the contamination with granulocytes, red blood cells and platelets is reduced to 7.2, 1.5% and 41%, respectively, of the cells initially present in the BM suspensions. Furthermore, it is demonstrated that this MNC concentrate is highly enriched in granulocyte-macrophage-colony-forming cells (CFU-GM; recovery 83%). The method is simple, inexpensive, efficient and reproducible. It allows rapid processing of a large volume of BM without substantial loss of hematopoietic progenitor cells. It represents a valuable method of BM MNC concentration prior to further in vitro manipulations such as T cell or tumor cell depletion or cryopreservation.
在本研究中，我们报告了使用连续流动细胞分离器Cobe 2997从人骨髓抽吸物中分离含造血干细胞的单核细胞（MNC）组分的评估结果。这种MNC浓缩物以原始骨髓体积的15%分离得到，包含初始有核细胞的23%。就骨髓MNC组分而言（淋巴细胞+单核细胞回收率；80%）它得到了富集，而粒细胞、红细胞和血小板的污染分别降至骨髓悬浮液中最初存在细胞的7.2%、1.5%和41%。此外，已证明这种MNC浓缩物富含粒细胞-巨噬细胞集落形成细胞（CFU-GM；回收率83%）。该方法简单、廉价、高效且可重复。它允许对大量骨髓进行快速处理，而造血祖细胞无大量损失。它是在进一步体外操作（如T细胞或肿瘤细胞清除或冷冻保存）之前进行骨髓MNC浓缩的一种有价值的方法。
