Figdor C G, de Witte T, Raymakers R, Haanen C, Bont W S
Vox Sang. 1985;49(2):154-60. doi: 10.1111/j.1423-0410.1985.tb00784.x.
A new type of blood component separator (BCS) was used for the isolation of hematopoietic progenitor cells from human bone marrow aspirates. The BCS was filled with 100-150 ml bone marrow and centrifuged to prepare a buffy coat. This buffy coat was isolated in 10-15% of the original bone marrow volume and contained 64 +/- 8% of the nucleated cells (NC). Morphological examination revealed that the buffy coat was highly enriched for myeloblasts, promyelocytes, lymphocytes and monocytes, whereas the contamination with granulocytes was reduced to 46 +/- 8% of the granulocytes initially present in the bone marrow suspension. In addition the contamination with red blood cells (RBC) was very low; the buffy coat contained only 6 +/- 2% of the RBC. Furthermore it was demonstrated by means of colony assays that the buffy coat was highly enriched for hematopoietic progenitor cells. It contained 91 +/- 6% of the granulocyte/monocyte progenitor cells (CFU-GM) and 87 +/- 9% of the erythroid progenitor cells (BFU-E). These results are comparable to those obtained with continuous or semicontinuous blood cell processors. The advantages of the BCS is that it is a simple and inexpensive apparatus which fits in a normal blood bank centrifuge. It permits efficient preparation and isolation of a buffy coat from human bone marrow without substantial loss of hematopoietic progenitor cells.
一种新型血液成分分离器（BCS）被用于从人骨髓抽吸物中分离造血祖细胞。该BCS中装入100 - 150毫升骨髓并进行离心以制备血沉棕黄层。该血沉棕黄层的体积为原始骨髓体积的10 - 15%，且含有64±8%的有核细胞（NC）。形态学检查显示，血沉棕黄层富含成髓细胞、早幼粒细胞、淋巴细胞和单核细胞，而粒细胞污染降至骨髓悬液中最初存在的粒细胞的46±8%。此外，红细胞（RBC）污染非常低；血沉棕黄层仅含6±2%的RBC。此外，通过集落测定表明，血沉棕黄层富含造血祖细胞。它含有91±6%的粒细胞/单核细胞祖细胞（CFU - GM）和87±9%的红系祖细胞（BFU - E）。这些结果与使用连续或半连续血细胞处理仪获得的结果相当。BCS的优点是它是一种简单且廉价的仪器，可适配于普通血库离心机。它能有效地从人骨髓中制备和分离血沉棕黄层，而不会造成造血祖细胞的大量损失。
