Tissue Culture Laboratory, Virus Research Institute, Pyongyang Medical College, KIM IL SUNG University, Pyongyang, Democratic People's Republic of Korea.
Tissue Culture Laboratory, Virus Research Institute, Pyongyang Medical College, KIM IL SUNG University, Pyongyang, Democratic People's Republic of Korea.
Cryobiology. 2019 Apr;87:115-116. doi: 10.1016/j.cryobiol.2019.01.017. Epub 2019 Jan 31.
The purpose of the present study was to clarify the possibility of a rapid cryopreservation for cell line Paesun by cooling in the range of 30-40 °C/min to vapor phase of -120 ∼-140 °C before immersion into liquid phase of liquid nitrogen using 10% MeSO. After thawing, these cells were examined with assaying viability by trypan blue exclusion staining and survival by cloning in monolayer; the percentages of cell and colony recovery obtained in rapid cooling had a tendency to be lower than that by slow cooling of 1 °C/min but there were no significant differences between them. In addition, post-thaw cells were examined by assaying proliferation and susceptibility to virus lines; there were no significant differences between before and after cryopreservation. In conclusion, these findings indicate that Paesun can be successfully cryopreserved by the rapid cooling rate of 30 °C-40 °C/min.
本研究旨在通过在 30-40°C/min 的范围内冷却到-120∼-140°C 的气相，然后浸入液氮的液相中，使用 10% MeSO 来澄清快速冷冻 Paesun 细胞系的可能性。解冻后，通过台盼蓝排斥染色法测定细胞活力和单层克隆法测定存活能力来检查这些细胞；快速冷却获得的细胞和集落回收率的百分比有低于 1°C/min 慢冷却的趋势，但两者之间没有显著差异。此外，还通过检测增殖能力和对病毒系的敏感性来检查解冻后的细胞；冷冻前后没有显著差异。总之，这些发现表明，Paesun 可以通过 30-40°C/min 的快速冷却速率成功冷冻保存。
