Tissue Culture Laboratory, Virus Research Institute, Pyongyang Medical College, KIM IL SUNG University, Pyongyang, Democratic People's Republic of Korea.
Tissue Culture Laboratory, Virus Research Institute, Pyongyang Medical College, KIM IL SUNG University, Pyongyang, Democratic People's Republic of Korea.
Cryobiology. 2020 Feb 1;92:258-259. doi: 10.1016/j.cryobiol.2019.11.003. Epub 2019 Nov 12.
The purpose of the present study was to assess the impact of cryoprotective media on cryopreservation of Paesun cells using loading trehalose. 30 mM trehalose was added after confluence of cells, and cultures were further incubated for 18 h at 37 °C. Cryoprotective media was "Cryocool" for the experiment, while MEM containing 10% FBS for the control. After thawing, these cells were examined with assaying the percentage of viable cells and the recovery rate; 89.2 ± 1.4% and 78.8 ± 3.2%, respectively in the experiment group, while 33.1 ± 2.9% and 21.5 ± 2.1%, respectively in the control group. Post-thaw cells of the experiment group were examined by assaying proliferation and susceptibility to virus lines; there were no significant differences between before and after cryopreservation, while cells of control group could not be recultured. In conclusion, the cryoprotective media impacts on the effectiveness of cryopreservation using loading trehalose.
本研究旨在评估使用海藻糖加载法对 Paesun 细胞进行冷冻保存时，冷冻保护剂对冷冻保存的影响。在细胞汇合后加入 30mM 的海藻糖，然后在 37°C 下进一步孵育 18 小时。实验中的冷冻保护剂为“Cryocool”，而对照中的则为含 10%FBS 的 MEM。解冻后，通过检测活细胞百分比和回收率来检查这些细胞；实验组分别为 89.2±1.4%和 78.8±3.2%，而对照组分别为 33.1±2.9%和 21.5±2.1%。通过检测增殖能力和对病毒系的敏感性来检查实验组解冻后的细胞，冷冻前后无显著差异，而对照组的细胞则无法再培养。总之，冷冻保护剂对使用海藻糖加载法的冷冻保存效果有影响。
