异硫氰酸盐在人恶性黑色素瘤（A375）细胞新暴露方案中诱导细胞周期停滞。
Isothiocyanate-induced Cell Cycle Arrest in a Novel Exposure Protocol of Human Malignant Melanoma (A375) Cells.
作者信息
Mantso Theodora, Anestopoulos Ioannis, Lamprianidou Eleftheria, Kotsianidis Ioannis, Pappa Aglaia, Panayiotidis Mihalis I
机构信息
Department of Applied Sciences, Northumbria University, Newcastle Upon Tyne, U.K.
Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece.
出版信息
Anticancer Res. 2019 Feb;39(2):591-596. doi: 10.21873/anticanres.13152.
BACKGROUND/AIM: Several studies have documented the effects of isothiocyanates (ITCs) on cancer prevention by inducing oxidative stress, activating apoptosis, affecting cell-cycle regulation, etc. Previously, we have shown that ITCs, administered at low concentrations by the means of double-bolus are capable of potentiating cytotoxicity in human malignant melanoma (A375) cells by inducing apoptosis. The aim of the present study was to further investigate the effect of the treatment of A375 cells with ITCs on cell-cycle progression and the levels of various cell cycle regulators.
MATERIALS AND METHODS
Cell-cycle analysis was performed by means of flow cytometry whereas western immunoblotting was used to determine the expression levels of these protein regulators.
RESULTS
Our data showed an increase in the number of cells in the G/M phase accompanied by a decrease in the G/G phase, while several cell-cycle regulators were shown to be differentially expressed upon exposure to ITCs.
CONCLUSION
ITCs induced cell-cycle arrest in A375 cells.
背景/目的：多项研究记录了异硫氰酸盐（ITCs）通过诱导氧化应激、激活凋亡、影响细胞周期调控等对癌症预防的作用。此前，我们已表明，通过双推注方式以低浓度给予ITCs能够通过诱导凋亡增强人恶性黑色素瘤（A375）细胞的细胞毒性。本研究的目的是进一步研究用ITCs处理A375细胞对细胞周期进程和各种细胞周期调节因子水平的影响。
材料与方法
通过流式细胞术进行细胞周期分析，而采用蛋白质免疫印迹法来确定这些蛋白质调节因子的表达水平。
结果
我们的数据显示，G/M期细胞数量增加，同时G/G期细胞数量减少，而暴露于ITCs后，几种细胞周期调节因子显示出差异表达。
结论
ITCs诱导A375细胞发生细胞周期阻滞。
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