Nakayama Koji, Seike Masahiro, Noro Rintaro, Takeuchi Susumu, Matsuda Kuniko, Kunugi Shinobu, Kubota Kaoru, Gemma Akihiko
Division of Pulmonary Medicine and Oncology, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan.
Division of Pulmonary Medicine and Oncology, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan
Anticancer Res. 2019 Feb;39(2):627-633. doi: 10.21873/anticanres.13156.
BACKGROUND/AIM: Malignant mesothelioma (MM) is an aggressive tumor with poor prognosis. The establishment of a new diagnostic and therapeutic approach for MM is expected. This study investigated the diagnostic significance of tenascin XB (TNXB) for MM.
TNXB gene expression was found to be significantly higher in MM tumor tissues compared to paired normal tissues, as assessed by the Gene Expression Omnibus database. The inhibition of TNXB using small interfering RNAs suppressed the proliferation and colony formation of MM cells. Expression of TNXB and calretinin, a current diagnostic marker of MM, was evaluated by immunohistochemistry.
The sensitivity and specificity of TNXB for MM were 80.0% and 69.5%, respectively. When the detection of TNXB was combined with that of calretinin, 83.3% of MM cases were detected.
These findings suggest that TNXB is a novel diagnostic biomarker for MM. A combination of detecting TNXB and calretinin may be useful for the differential diagnosis of MM from lung adenocarcinoma.
背景/目的：恶性间皮瘤（MM）是一种侵袭性肿瘤，预后较差。期望建立一种新的MM诊断和治疗方法。本研究调查了腱生蛋白XB（TNXB）对MM的诊断意义。
通过基因表达综合数据库评估发现，与配对的正常组织相比，MM肿瘤组织中TNXB基因表达显著更高。使用小干扰RNA抑制TNXB可抑制MM细胞的增殖和集落形成。通过免疫组织化学评估TNXB和钙结合蛋白（MM目前的诊断标志物）的表达。
TNXB对MM的敏感性和特异性分别为80.0%和69.5%。当TNXB检测与钙结合蛋白检测相结合时，83.3%的MM病例被检测出来。
这些发现表明TNXB是MM的一种新型诊断生物标志物。联合检测TNXB和钙结合蛋白可能有助于MM与肺腺癌的鉴别诊断。
