Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha, 769008, India.
Environ Sci Pollut Res Int. 2019 Nov;26(33):34277-34284. doi: 10.1007/s11356-019-04304-0. Epub 2019 Feb 2.
Lipase, a versatile hydrolytic enzyme, is gaining more importance in environmental applications such as treatment of oil and grease containing wastewater, pretreatment of solid waste/industrial wastewater for anaerobic treatment. In the present study, the attempts have been made to improve the production of lipase from Staphylococcus hominis MTCC 8980 by optimization of pH, temperature, and agitation speed in lab scale shake flasks culture. The experiments were designed using the full factorial central composite design of experiment. A total of 20 experiments were conducted, and the optimized pH, temperature, and agitation speed were found to be 7.9, 33.1 °C, and 178.4 rpm, respectively. The results of the analysis of variance (ANOVA) test revealed that the linear terms for temperature and agitation were significant (p value < 0.05). Interaction for pH and agitation speed was found to have a significant effect on lipase production from S. hominis MTCC 8980. A 150% increase in enzyme activity was observed under the optimized conditions with the maximum lipase activity of 1.82 U/ml. Further enhancement of enzyme activity can be expected from the optimization of medium components.
脂肪酶是一种多功能的水解酶，在环境应用中越来越重要，例如处理含油和油脂的废水、预处理固体废物/工业废水以进行厌氧处理。在本研究中，尝试通过优化摇瓶培养中的 pH、温度和搅拌速度来提高金黄色葡萄球菌 MTCC 8980 脂肪酶的产量。实验采用全因子中心组合设计实验设计。共进行了 20 次实验，优化的 pH、温度和搅拌速度分别为 7.9、33.1°C 和 178.4 rpm。方差分析（ANOVA）测试的结果表明，温度和搅拌的线性项具有显著影响（p 值 < 0.05）。发现 pH 和搅拌速度的相互作用对金黄色葡萄球菌 MTCC 8980 脂肪酶的生产有显著影响。在优化条件下，酶活性增加了 150%，最大脂肪酶活性达到 1.82 U/ml。通过优化培养基成分，预计可以进一步提高酶活性。
