a Pulmonary and Critical Care Medicine, Department of Medicine , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
b Pulmonary and Critical Care Medicine, Department of Medicine and Marsico Lung Institute , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina , USA.
COPD. 2018 Dec;15(6):572-580. doi: 10.1080/15412555.2019.1566892. Epub 2019 Feb 4.
Mucus hydration is important in mucus clearance and lung health. This study sought to test the relative utility of spontaneous sputum (SS) versus the reasonably noninvasive induced sputum (IS) samples for measurement of mucus hydration. SS and IS samples were collected over a 2-day study interval. Sputum was induced with escalating inhaled nebulized 3-5% hypertonic saline. Viscous portions of the samples ("plugs") were utilized for percent solids and total mucin analyses. Cytokines, nucleotides/nucleosides and cell differentials were measured in plugs diluted into 0.1% Sputolysin. Overall, 61.5% of chronic bronchitis (CB) subjects produced a SS sample and 95.2% an IS sample. Total expectorate sample weights were less for the SS (0.94 ± 0.98 g) than the IS (2.67 ± 2.33 g) samples. Percent solids for the SS samples (3.56% ± 1.95; n = 162) were significantly greater than the IS samples (3.08% ± 1.81; n = 121), p = 0.133. Total mucin concentrations also exhibited a dilution of the IS samples: SS = 4.15 ± 3.23 mg/ml (n = 62) versus IS= 3.34 ± 2.55 mg/ml (n = 71) (p = 0.371). Total mucins (combined SS and IS) but not percent solids, were inversely associated with FEV percent predicted (p = 0.052) and FEV,/FVC % (p = 0.035). There were no significant differences between sample types in cytokine or differential cell counts. The probability of sample collections was less for SS than IS samples. Measurements of hydration revealed modest dilution of the IS samples compared to SS. Thus for measurements of mucus hydration, both SS and IS samples appear to be largely interchangeable.
黏液水合作用对黏液清除和肺部健康很重要。本研究旨在测试自然咳痰（SS）与合理非侵入性诱导咳痰（IS）样本在测量黏液水合作用方面的相对效用。在为期两天的研究间隔内收集 SS 和 IS 样本。通过逐渐吸入雾化的 3-5%高渗盐水来诱导咳痰。利用样本的粘性部分（“塞子”）进行固体百分比和总粘蛋白分析。在稀释至 0.1%Sputolysin 的塞子中测量细胞因子、核苷酸/核苷和细胞差异。总体而言，61.5%的慢性支气管炎（CB）患者产生 SS 样本，95.2%的患者产生 IS 样本。SS（0.94±0.98g）的总咳出物样本重量明显低于 IS（2.67±2.33g）样本。SS 样本的固体百分比（3.56%±1.95；n=162）明显高于 IS 样本（3.08%±1.81；n=121），p=0.133。IS 样本的总粘蛋白浓度也出现了稀释：SS=4.15±3.23mg/ml（n=62）与 IS=3.34±2.55mg/ml（n=71）（p=0.371）。总粘蛋白（SS 和 IS 总和）但不是固体百分比与 FEV%预测值（p=0.052）和 FEV/FVC%（p=0.035）呈反比。两种样本类型在细胞因子或差异细胞计数方面无显著差异。SS 样本的采集概率低于 IS 样本。与 SS 样本相比，IS 样本的水合作用测量结果略有稀释。因此，对于黏液水合作用的测量，SS 和 IS 样本似乎可以互换使用。
