Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; International Corporation Laboratory of Agriculture and Agricultural Products Safety, Yangzhou University, Yangzhou 225009, Jiangsu, China.
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China.
J Dairy Sci. 2019 Apr;102(4):3469-3473. doi: 10.3168/jds.2018-15481. Epub 2019 Feb 1.
Bovine leukemia virus (BLV) causes enzootic leucosis in cattle and is classified into 10 genotypes with a worldwide distribution, except for several European countries, Australia, and New Zealand. Although BLV is widespread in Chinese cows with the positive rate of 49.1% at the individual level, very little is known about the BLV genotype in dairy cattle in China. To determine BLV genetic variability in cows in China, 112 BLV-positive samples from 5 cities in China were used for BLV molecular characterization in this study. Phylogenetic analysis using the neighbor-joining method on partial env sequence encoding gp51 obtained from 5 Chinese cities and those available in GenBank (n = 53, representing BLV genotype 1-10) revealed the Chinese strains belonged to genotype 6. Seven unique SNP were identified among Yancheng, Shanghai, and Bengbu strains out of the total 12 SNP identified in Chinese strains. The genotyping coupled with SNP analysis of BLV can serve as a useful molecular epidemiological tool for tracing the source of pathogens. This study highlights the importance of genetic analysis of geographically diverse BLV strains to understand BLV global genetic diversity.
牛白血病病毒（BLV）可引起牛地方性白血病，该病毒分为 10 种基因型，在世界范围内广泛分布，除了一些欧洲国家、澳大利亚和新西兰。尽管 BLV 在我国奶牛中广泛存在，个体阳性率为 49.1%，但对我国奶牛 BLV 基因型知之甚少。为了确定中国奶牛中的 BLV 遗传变异，本研究使用了来自中国 5 个城市的 112 个 BLV 阳性样本进行 BLV 分子特征分析。对来自中国 5 个城市的 53 个 BLV 基因型 1-10 的 env 序列编码 gp51 的部分序列进行邻接法系统进化分析，结果表明中国分离株属于基因型 6。在总共鉴定出的 12 个 SNP 中，盐城、上海和蚌埠株有 7 个独特的 SNP。对 BLV 的基因分型和 SNP 分析可以作为追踪病原体来源的有用分子流行病学工具。本研究强调了对地理分布不同的 BLV 株进行遗传分析，以了解 BLV 全球遗传多样性的重要性。
