Nobushi Yasuhito, Asamoto Hiromichi, Uchikura Kazuo
School of Pharmacy, Nihon University.
College of Industrial Technology, Nihon University.
Yakugaku Zasshi. 2019;139(2):309-315. doi: 10.1248/yakushi.18-00082.
We developed a flow injection analysis (FIA) method based on tris(2,2'-bipyridine)ruthenium(III) [Ru(bpy) ] electrogenerated chemiluminescence for assessment of antioxidant property. The hydroxyl radical (∙OH) were generated by HO photolysis using an ultraviolet/HO photoreactor. The reactor comprised a polytetrafluoroethylene tube, a quartz container, and a low-pressure mercury lamp that predominantly emitted radiation at around 185 nm. When the hydroxyl radical and Ru(bpy) were in contact, chemiluminescence was generated as background emission. The background emission decreased when antioxidant samples were injected to the system. The antioxidant property of the naturally occurring antioxidants tested are listed herein, starting with the highest: gallic acid>ascorbic acid>quercetin. Moreover, our method allowed a sample throughput of approximately 100 samples/h. The proposed high throughput method can be used to assess the antioxidant property of the naturally occurring antioxidants.
我们开发了一种基于三（2,2'-联吡啶）钌（III）[Ru(bpy)₃] 电致化学发光的流动注射分析（FIA）方法，用于评估抗氧化性能。使用紫外/过氧化氢光反应器通过过氧化氢光解产生羟基自由基（∙OH）。该反应器包括一个聚四氟乙烯管、一个石英容器和一个主要发射波长约为185nm辐射的低压汞灯。当羟基自由基与Ru(bpy)₃接触时，会产生化学发光作为背景发射。当将抗氧化剂样品注入系统时，背景发射会降低。本文列出了所测试的天然抗氧化剂的抗氧化性能，从最高的开始：没食子酸>抗坏血酸>槲皮素。此外，我们的方法允许每小时大约处理100个样品。所提出的高通量方法可用于评估天然抗氧化剂的抗氧化性能。
