Nobushi Yasuhito, Uchikura Kazuo
College of Pharmacy, Nihon University, Chiba, Japan.
Chem Pharm Bull (Tokyo). 2010 Jan;58(1):117-20. doi: 10.1248/cpb.58.117.
We describe a new method for detecting hydroxyl radical scavenging capacity based on tris(2,2'-bipyridine)ruthenium(III) [Ru(bpy)(3)(3+)] chemiluminescence and flow injection analysis. Hydroxyl radicals were generated by the Fenton reaction. The scavenging capacity of the six antioxidants tested decreased in the following order: edaravone>L-tryptophan>gallic acid>Trolox>N-acetyl-L-cysteine>ascorbic acid. The proposed method allowed a sample throughput of about 80 samples/h. The six antioxidants were found to inhibit chemiluminescence intensity of Ru(bpy)(3)(2+). The proposed method is a rapid, selective, and accurate procedure for the study of hydroxyl radical scavenging capacity by Ru(bpy)(3)(3+) chemiluminescence.
我们描述了一种基于三（2,2'-联吡啶）钌（III）[Ru(bpy)(3)(3+)]化学发光和流动注射分析检测羟自由基清除能力的新方法。羟自由基通过芬顿反应产生。所测试的六种抗氧化剂的清除能力按以下顺序降低：依达拉奉>L-色氨酸>没食子酸> Trolox>N-乙酰-L-半胱氨酸>抗坏血酸。所提出的方法允许每小时约80个样品的通量。发现这六种抗氧化剂抑制Ru(bpy)(3)(2+)的化学发光强度。所提出的方法是一种通过Ru(bpy)(3)(3+)化学发光研究羟自由基清除能力的快速、选择性和准确的程序。
