Narenji Sani Reza, Hajigolikhani Bahram, Ahmadi-Hamedani Mahmood, Kafshdouzan Khatereh
Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Vet Res Forum. 2018 Fall;9(4):343-348. doi: 10.30466/vrf.2018.33077. Epub 2018 Dec 15.
The aim of the present study was to investigate the diagnostic evaluation of milk lactate dehydrogenase (mLDH) and alkaline phosphatase (mALP) activities by receiver operating characteristic (ROC) analysis curve in early lactation of ewes with subclinical mastitis (SCM) and determine the correlation between number of somatic cell count (SCC) and mLDH and mALP activities. A total of 196 udder half milk samples were collected within the first 6 weeks of lambing. The SCM was determined by positive milk bacterial culture and positive California mastitis test (CMT); SCC was determined by fossomatic method and enzyme activities were determined spectrophotometrically. The mLDH and mALP of SCM cases were positively correlated with SCC values. Values of mLDH, mALP and SCC were significantly higher in SCM than non-SCM udder halves. The optimum cut-off points of mLDH and mALP activities for SCM diagnosis were determined at 203.61 (U L) and 329.84 (U L), respectively. In conclusion, SCC has positive correlation with mALP and mLDH activities in SCM ewes and mLDH and mALP activities could be considered as reliable indicators for intra-mammary inflammation diagnosis.
本研究的目的是通过受试者工作特征（ROC）分析曲线，调查亚临床型乳房炎（SCM）母羊泌乳早期乳中乳酸脱氢酶（mLDH）和碱性磷酸酶（mALP）活性的诊断评估，并确定体细胞计数（SCC）与mLDH和mALP活性之间的相关性。在产羔后的前6周内，共采集了196份半乳区奶样。通过阳性奶细菌培养和阳性加利福尼亚乳房炎检测（CMT）确定SCM；通过福斯马托法测定SCC，并通过分光光度法测定酶活性。SCM病例的mLDH和mALP与SCC值呈正相关。SCM的mLDH、mALP和SCC值显著高于非SCM半乳区。SCM诊断的mLDH和mALP活性的最佳截断点分别确定为203.61（U/L）和329.84（U/L）。总之，SCC与SCM母羊的mALP和mLDH活性呈正相关，mLDH和mALP活性可被视为乳腺内炎症诊断的可靠指标。
