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胡齐斯坦水牛的细胞遗传学鉴定

Cytogenetic identification of Khuzestani water Buffalo.

作者信息

Alikhani Javad, Mohammadi Godratollah, Shariati Gholamreza

机构信息

Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Department of Genetics, Faculty of Medicine, Jundishapur University of Medical Sciences, Ahvaz, Iran.

出版信息

Vet Res Forum. 2018 Fall;9(4):357-360. doi: 10.30466/vrf.2018.33075. Epub 2018 Dec 15.

Abstract

The domestic water buffalo (Bubalus bubalis) is an economically important livestock species in many Asian and Mediterranean countries. Two types of water buffalos are river and swamp buffaloes. Genetically, the swamp buffalo has 48 chromosomes (2n = 48) and the river buffalo has 50 chromosomes (2n = 50). Khuzestan province is one of the important centers of buffalo farming in Iran. Since there was no cytogenetic information about Khuzestani buffalo breed, the present study was performed to provide karyotype and cytogenetic study in Khuzestan buffaloes. In this study, blood samples of 40 buffaloes were collected randomly from jugular vein via heparinized syringes. For metaphase smear, blood samples were cultured in RPMI 1640 medium containing fetal bovine serum and phitohemaglutination and then transferred to water bath for 69 hr. After stopping cell division by colcemide and using fixed solution, from each sample six slides were stained with Giemsa staining. Based on the results, number of chromosomes of the Khuzestani buffaloes was 50 (2n = 50). Among the 25 pairs of chromosomes, the first 5 pairs of autosomal chromosomes were sub-metacentric, 19 pairs of autosomal ones were acrocentric and the sex chromosomes (X and Y) were acrocentric. Furthermore, X and Y chromosomes were the largest and smallest acrocentric ones, respectively. Therefore, the buffaloes of Khuzestan province are considered as a river type. ‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬.

摘要

家养水牛(Bubalus bubalis)在许多亚洲和地中海国家都是具有重要经济价值的家畜品种。水牛主要分为河流型和沼泽型两种。从基因角度来看,沼泽型水牛有48条染色体(2n = 48),而河流型水牛有50条染色体(2n = 50)。胡齐斯坦省是伊朗重要的水牛养殖中心之一。由于此前没有关于胡齐斯坦水牛品种的细胞遗传学信息,因此开展了本研究,以对胡齐斯坦水牛进行核型分析和细胞遗传学研究。在本研究中,通过肝素化注射器从40头水牛的颈静脉随机采集血样。对于中期涂片,将血样在含有胎牛血清和植物血凝素的RPMI 1640培养基中培养,然后转移至水浴中培养69小时。在用秋水仙胺终止细胞分裂并使用固定液后,从每个样本制作六张玻片,用吉姆萨染色法染色。根据结果,胡齐斯坦水牛的染色体数目为50条(2n = 50)。在25对染色体中,前5对常染色体为亚中着丝粒染色体,19对常染色体为近端着丝粒染色体,性染色体(X和Y)为近端着丝粒染色体。此外,X和Y染色体分别是最大和最小的近端着丝粒染色体。因此,胡齐斯坦省的水牛被认为是河流型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a5f/6346483/ce80080d6d80/vrf-9-357-g001.jpg

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