Department of Oral Pathology and Microbiology, YMT Dental College and Hospital, Navi Mumbai, India.
Department of Oral and Maxillofacial Pathology and Microbiology, Saveetha Dental College and Hospital, Chennai, India.
J Oral Pathol Med. 2019 Apr;48(4):299-306. doi: 10.1111/jop.12835. Epub 2019 Feb 18.
Metabolomics is the study of metabolome which describes the full repertoire of small molecules, and the analysis of salivary metabolomics may help in identifying tumor-specific biomarkers for early diagnosis and prediction of tumor progression. The aim of the study was to evaluate the clinical utility of salivary metabolites in oral leukoplakia and oral squamous cell carcinoma.
Salivary metabolomic profile of patients diagnosed with oral leukoplakia (n = 21) and oral squamous cell carcinoma (n = 22) was compared with apparently normal controls (n = 18) using Q-TOF-liquid chromatography-mass spectrometry. MassHunter profile software and Metlin database were used for metabolite identification. ANOVA to identify the regulation of metabolites between the three groups, t test (P < 0.05) to signify the changes between two groups, and chi-square test (P < 0.05) to indicate the presence or absence of metabolites in the study participants of the three groups were performed.
Significant upregulation of 1-methylhistidine, inositol 1,3,4-triphosphate, d-glycerate-2-phosphate, 4-nitroquinoline-1-oxide, 2-oxoarginine, norcocaine nitroxide, sphinganine-1-phosphate, and pseudouridine in oral leukoplakia and OSCC was noted. Downregulated compounds in the diseased groups included l-homocysteic acid, ubiquinone, neuraminic acid, and estradiol valerate.
A range of salivary metabolites were significantly altered in oral leukoplakia and oral squamous cell carcinoma. Further, it is necessary to evaluate the clinical utility of the individual metabolites in preventing malignant transformation of oral leukoplakia and to improve prognosis of oral squamous cell carcinoma.
代谢组学是研究代谢组的，它描述了小分子的全部谱，分析唾液代谢组学可能有助于发现肿瘤特异性生物标志物，用于早期诊断和预测肿瘤进展。本研究旨在评估唾液代谢物在口腔白斑病和口腔鳞状细胞癌中的临床应用价值。
采用 Q-TOF 液相色谱-质谱联用技术比较了 21 例口腔白斑病患者、22 例口腔鳞状细胞癌患者和 18 例貌似正常对照者的唾液代谢组图谱。使用 MassHunter profile 软件和 Metlin 数据库进行代谢物鉴定。采用方差分析（ANOVA）鉴定三组间代谢物的调控，采用 t 检验（P<0.05）比较两组间的变化，采用卡方检验（P<0.05）判断三组研究参与者中代谢物的存在或缺失。
在口腔白斑病和口腔鳞状细胞癌中，1-甲基组氨酸、肌醇 1,3,4-三磷酸、d-甘油酸-2-磷酸、4-硝基喹啉-1-氧化物、2-氧代精氨酸、去甲可卡因氮氧化物、鞘氨醇 1-磷酸和假尿嘧啶显著上调。在病变组中下调的化合物包括 l-高半胱氨酸、泛醌、神经氨酸和戊酸雌二醇。
在口腔白斑病和口腔鳞状细胞癌中，多种唾液代谢物发生了显著改变。进一步评估个体代谢物在预防口腔白斑病恶性转化和改善口腔鳞状细胞癌预后方面的临床应用价值是必要的。
