Sridharan Gokul, Ramani Pratibha, Patankar Sangeeta
Department of Oral Pathology and Microbiology, YMT Dental College and Hospital, Navi Mumbai, Maharashtra, India.
Department of Oral Pathology and Microbiology, Saveetha Dental College and Hospitals, Saveetha University, Chennai, Tamil Nadu, India.
J Cancer Res Ther. 2017 Jul-Sep;13(3):556-561. doi: 10.4103/jcrt.JCRT_1233_16.
Metabolomics is a core discipline of system biology focusing on the study of low molecular weight compounds in biological system. Analysis of human metabolome, which is composed of diverse group of metabolites, can aid in diagnosis and prognosis of oral squamous cell carcinoma (OSCC).
The aim of the present study is to analyze and identify serum metabolites in oral leukoplakia and OSCC as a potential diagnostic biomarker and a predictor for malignant transformation of oral leukoplakia.
Serum metabolomic profile of patients diagnosed with oral leukoplakia (n = 21) and OSCC (n = 22) was compared with normal controls (n = 18) using quadrupole time of flight-liquid chromatography-mass spectrometry. MassHunter profile software was used for metabolite identification, and statistical analysis to assess the variation of the metabolites was performed using Mass Profiler Professional software. Statistical significance between the three groups was expressed using ANOVA (P < 0.05), and intergroup comparison was done using Student's t-test (P < 0.05).
Significant upregulation of estradiol-17-beta-3-sulfate, L-carnitine, 5-methylthioadenosine (MTA), 8-hydroxyadenine, 2-methylcitric acid, putrescine, and estrone-3-sulfate was seen in oral leukoplakia and OSCC than in normal controls. Furthermore, significant upregulation of 5,6-dihydrouridine, 4-hydroxypenbutolol glucuronide, 8-hydroxyadenine, and putrescine was evident in OSCC group than in oral leukoplakia.
Upregulation of L-carnitine, lysine, 2-methylcitric acid, putrescine; 8-hydroxyadenine; 17-estradiol; 5,6-dihydrouridine; and MTA suggests their diagnostic potential in oral leukoplakia and OSCC. Further, a significant upregulation of putrescine, 8-hydroxyadenine, and 5,6-dihydrouridine in OSCC than in oral leukoplakia indicates their potential role in predicting the malignant transformation of oral leukoplakia.
代谢组学是系统生物学的核心学科，专注于研究生物系统中的低分子量化合物。对由多种代谢物组成的人类代谢组进行分析，有助于口腔鳞状细胞癌（OSCC）的诊断和预后评估。
本研究的目的是分析和鉴定口腔白斑和口腔鳞状细胞癌患者血清中的代谢物，作为潜在的诊断生物标志物以及口腔白斑恶变的预测指标。
采用四极杆飞行时间液相色谱 - 质谱联用技术，将诊断为口腔白斑（n = 21）和口腔鳞状细胞癌（n = 22）患者的血清代谢组学图谱与正常对照组（n = 18）进行比较。使用MassHunter图谱软件进行代谢物鉴定，并使用Mass Profiler Professional软件进行统计分析以评估代谢物的变化。三组间的统计学显著性用方差分析表示（P < 0.05），组间比较采用学生t检验（P < 0.05）。
与正常对照组相比，口腔白斑和口腔鳞状细胞癌患者中雌二醇 - 17 - β - 3 - 硫酸盐、L - 肉碱、5 - 甲硫腺苷（MTA）、8 - 羟基腺嘌呤、2 - 甲基柠檬酸、腐胺和雌酮 - 3 - 硫酸盐有显著上调。此外，与口腔白斑组相比，口腔鳞状细胞癌组中5,6 - 二氢尿苷、4 - 羟基喷布洛尔葡萄糖醛酸、8 - 羟基腺嘌呤和腐胺有显著上调。
L - 肉碱、赖氨酸、2 - 甲基柠檬酸、腐胺、8 - 羟基腺嘌呤、17 - 雌二醇、5,6 - 二氢尿苷和MTA的上调表明它们在口腔白斑和口腔鳞状细胞癌中的诊断潜力。此外，与口腔白斑相比，口腔鳞状细胞癌中腐胺、8 - 羟基腺嘌呤和5,6 - 二氢尿苷的显著上调表明它们在预测口腔白斑恶变中的潜在作用。
