Polonis Marcin, Jagiełło Krzysztof, Dobosz Stefan, Rożyński Rafał, Kondraciuk Paulina, Gurgul Artur, Szmatoła Tomasz, Ocalewicz Konrad
Department of Marine Biology and Ecology, Faculty of Oceanography and Geography, Institute of Oceanography, University of Gdansk, Gdynia, Poland.
Department of Salmonid Research, Inland Fisheries Institute in Olsztyn, Rutki, Poland.
Reprod Domest Anim. 2019 Apr;54(4):712-718. doi: 10.1111/rda.13413. Epub 2019 Feb 22.
Ionizing radiation (IR) is applied to inactivate nuclear genome in the salmonid eggs to induce androgenetic development. However, it has been considered that doses of IR used to damage maternal chromosomes may also affect morphology of the eggs and decrease their developmental potential. Thus, the main goal of the present research was to assess alterations in the rainbow trout (Oncorhynchus mykiss) eggs caused by the high dose of IR administered during androgenesis. In the present research, rainbow trout eggs were irradiated with 350 Gy of X-rays, inseminated and exposed to the high hydrostatic pressure (HHP) shock to develop as androgenetic doubled haploids (DHs). The distribution of lipid droplets in the irradiated and non-irradiated rainbow trout eggs, survival rates and morphology of larvae from androgenetic and control groups were compared. It has been observed that non-irradiated and irradiated eggs exhibited altered distribution of lipid droplets. Most of the eggs before IR treatment displayed rather equal distribution of the oil droplets. In turn, majority of eggs studied after irradiation had coalesced lipid droplets, a pattern found in eggs with reduced quality. Incidences of abnormally developed larvae were more frequently observed among fish that hatched from the irradiated eggs. Observed changes suggest X-rays applied for the genetic inactivation of rainbow trout eggs may lead to decrease of their developmental competence.
电离辐射（IR）被用于使鲑科鱼卵中的核基因组失活，以诱导雄核发育。然而，人们认为用于损伤母本染色体的电离辐射剂量可能也会影响鱼卵的形态，并降低其发育潜力。因此，本研究的主要目标是评估在雄核发育过程中高剂量电离辐射对虹鳟（Oncorhynchus mykiss）鱼卵造成的变化。在本研究中，虹鳟鱼卵用350戈瑞的X射线进行辐照，受精后再经受高静水压（HHP）冲击，以发育成雄核发育的双单倍体（DHs）。比较了辐照和未辐照的虹鳟鱼卵中脂滴的分布、雄核发育组和对照组幼虫的存活率及形态。据观察，未辐照和辐照后的鱼卵均呈现出脂滴分布的改变。大多数在电离辐射处理前的鱼卵显示出油滴分布较为均匀。相反，辐照后研究的大多数鱼卵出现了脂滴合并的情况，这种模式在质量下降的鱼卵中可见。在从辐照后的鱼卵孵化出的鱼苗中，更频繁地观察到发育异常的幼虫。观察到的变化表明，用于虹鳟鱼卵遗传失活的X射线可能会导致其发育能力下降。
