Jagiełło Krzysztof, Dobosz Stefan, Zalewski Tomasz, Polonis Marcin, Ocalewicz Konrad
Faculty of Oceanography and Geography, Department of Marine Biology and Ecology, Institute of Oceanography, University of Gdansk, Gdynia, Poland.
Department of Salmonid Research, Inland Fisheries Institute in Olsztyn, Żukowo, Poland.
Reprod Domest Anim. 2018 Oct;53(5):1176-1183. doi: 10.1111/rda.13223. Epub 2018 Jun 28.
Poor quality eggs produced by the fully homozygous doubled haploids (DHs) may impair generation of clonal lines in fish species. In the present research, gynogenetic development of rainbow trout (Oncorhynchus mykiss) was induced in eggs originated from the DH females. Eggs were activated with the UV-irradiated grayling (Thymallus thymallus) spermatozoa and subjected to the high hydrostatic pressure (HHP) shock to provide diploid clonal individuals. Only two of four DH females produced eggs that were successfully activated by the irradiated spermatozoa and subsequently developed into the gynogenetic embryos. Survival rates of rainbow trout from the clonal lines equalled 21.5% and 19.8% during embryogenesis and decreased after hatching to 18.6% and 14.9%, respectively. Some of the dead rainbow trout clones collected between hatching and swim-up stage were emaciated and exhibited spinal deformities including scoliosis. Provided results confirmed limited developmental competences of eggs produced by rainbow trout DH females. Clonal rainbow trout developing in such eggs exhibited reduced survival and increased frequency of the body abnormalities.
完全纯合的双单倍体（DHs）产生的劣质卵可能会损害鱼类克隆系的产生。在本研究中，对源自DH雌性的虹鳟（Oncorhynchus mykiss）卵进行了雌核发育诱导。用紫外线照射的茴鱼（Thymallus thymallus）精子激活卵，并施加高静水压（HHP）休克以产生二倍体克隆个体。四个DH雌性中只有两个产生的卵被照射的精子成功激活，并随后发育成雌核发育胚胎。克隆系虹鳟在胚胎发育期间的存活率分别为21.5%和19.8%，孵化后分别降至18.6%和14.9%。在孵化和上浮阶段之间收集的一些死亡虹鳟克隆个体瘦弱，并表现出包括脊柱侧弯在内的脊柱畸形。所提供的结果证实了虹鳟DH雌性产生的卵的发育能力有限。在这种卵中发育的克隆虹鳟存活率降低，身体异常频率增加。
