血液中的线粒体常见缺失水平：对年龄和体重指数影响的新见解
Mitochondrial Common Deletion Level in Blood: New Insight Into the Effects of Age and Body Mass Index.
作者信息
Ahmadi Mahboube, Golalipour Masoud, Samaei Nader M
机构信息
Department of Biology, School of Basic Sciences, Golestan University, Gorgan, Iran.
Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
出版信息
Curr Aging Sci. 2019;11(4):250-254. doi: 10.2174/1874609812666190201163421.
BACKGROUND
Age-related decrease in mitochondrial activity has been reported in several tissues. Reactive Oxygen Species (ROS) produced from defected mitochondria lead to aging and accumulate through time. However, studies about the mitochondrial DNA mutation level in blood are contradictory. Other lifestyle factors may modify the effects of age in post-mitotic tissues such as blood. The BMI represents the sum of the various lifestyle factors.
OBJECTIVE
We proposed that age, obesity and mtDNA deletion are three ROS producing factors, which may interact with each other and induce senescence.
METHODS
In a cross-sectional study, 172 male and female volunteers without known mitochondrial diseases were selected and the presence of common mitochondrial 4977bp deletion (ΔmtDNA4977) evaluated using Nested-PCR.
RESULTS
Our results showed that a high percentage of samples (54.06%) harbor common deletion in blood. Furthermore, both BMI and the ΔmtDNA4977 levels significantly decrease with age. The chronological age, BMI and ΔmtDNA4977 reciprocally affect each other.
CONCLUSION
Our data suggest that age affects purifying selection and BMI, which may influence the relative level of the mtDNA common deletion in blood.
背景
已有报道称，多种组织中都存在与年龄相关的线粒体活性下降。线粒体缺陷产生的活性氧（ROS）会导致衰老，并随时间积累。然而，关于血液中线粒体DNA突变水平的研究结果相互矛盾。其他生活方式因素可能会改变年龄对血液等有丝分裂后组织的影响。体重指数（BMI）代表了各种生活方式因素的总和。
目的
我们提出年龄、肥胖和线粒体DNA缺失是三个产生ROS的因素，它们可能相互作用并诱导衰老。
方法
在一项横断面研究中，选取了172名无已知线粒体疾病的男性和女性志愿者，采用巢式PCR评估常见线粒体4977bp缺失（ΔmtDNA4977）的存在情况。
结果
我们的结果显示，高比例的样本（54.06%）血液中存在常见缺失。此外，BMI和ΔmtDNA4977水平均随年龄显著下降。实际年龄、BMI和ΔmtDNA4977相互影响。
结论
我们的数据表明，年龄影响纯化选择和BMI，这可能会影响血液中线粒体DNA常见缺失的相对水平。
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