Zhang You-Fang
Zhejiang Police College, Hangzhou 310053, China.
Fa Yi Xue Za Zhi. 2007 Dec;23(6):438-40.
To explore depletion of human mitochondrial DNA 4977-bp and its relation with aging.
Total DNA (nuclear and mtDNA) was extracted from 100mg muscle tissue. UV light illumination of ethidium bromide-stained PCR products was used to study the depletion of mtDNA (wild-type or mutant).
The proportions of mtDNA depletion in human skeletal muscle could be determined. The frequency of mtDNA 4977-bp depletion in different age groups (0-9, 10-19, 20-29, 30-39, 40-49, 50-59, 60-69, 70-79, 80-89, 90-99) was: 0%, 0%, 0.003%, 0.011%, 0.015%, 0.033%, 0.038%, 0.062%, 0.069%, and 0.091%, respectively.
Our findings suggest that the frequency of the mtDNA4977 depletion in human skeletal muscle increases with age. It might be useful for human age estimation.
探讨人类线粒体DNA 4977碱基对缺失情况及其与衰老的关系。
从100mg肌肉组织中提取总DNA（核DNA和线粒体DNA）。用紫外线照射溴化乙锭染色的PCR产物来研究线粒体DNA（野生型或突变型）的缺失情况。
可确定人类骨骼肌中线粒体DNA缺失的比例。不同年龄组（0 - 9岁、10 - 19岁、20 - 29岁、30 - 39岁、40 - 49岁、50 - 59岁、60 - 69岁、70 - 79岁、80 - 89岁、90 - 99岁）中线粒体DNA 4977碱基对缺失的频率分别为：0%、0%、0.003%、0.011%、0.015%、0.033%、0.038%、0.062%、0.069%和0.091%。
我们的研究结果表明，人类骨骼肌中线粒体DNA 4977缺失的频率随年龄增长而增加。这可能对人类年龄估计有用。
