Nolasco Pedro, Coelho Paulo V, Coelho Carla, Angelo David F, Dias J R, Alves Nuno M, Maurício António, Pereira Manuel F C, Alves de Matos António P, Martins Raul C, Carvalho Patrícia A
CeFEMA, Instituto Superior Técnico, University of Lisbon,Av. Rovisco Pais, 1049-001 Lisboa,Portugal.
Service of Maxillofacial Surgery,Centro Hospitalar de Lisboa Central,R. José António Serrano 1150-199 Lisboa,Portugal.
Microsc Microanal. 2019 Feb;25(1):151-163. doi: 10.1017/S1431927618016124. Epub 2019 Feb 4.
The fraction of organic matter present affects the fragmentation behavior of sialoliths; thus, pretherapeutic information on the degree of mineralization is relevant for a correct selection of lithotripsy procedures. This work proposes a methodology for in vivo characterization of salivary calculi in the pretherapeutic context. Sialoliths were characterized in detail by X-ray computed microtomography (μCT) in combination with atomic emission spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. Correlative analysis of the same specimens was performed by in vivo and ex vivo helical computed tomography (HCT) and ex vivo μCT. The mineral matter in the sialoliths consisted essentially of apatite (89 vol%) and whitlockite (11 vol%) with average density of 1.8 g/cm3. In hydrated conditions, the mineral mass prevailed with 53 ± 13 wt%, whereas the organic matter, with a density of 1.2 g/cm3, occupied 65 ± 10% of the sialoliths' volume. A quantitative relation between sialoliths mineral density and X-ray attenuation is proposed for both HCT and μCT.
存在的有机物比例会影响涎石的碎裂行为；因此，治疗前关于矿化程度的信息对于正确选择碎石手术至关重要。这项工作提出了一种在治疗前对唾液结石进行体内表征的方法。通过X射线计算机显微断层扫描（μCT）结合原子发射光谱、傅里叶变换红外光谱、X射线衍射、扫描电子显微镜和透射电子显微镜对涎石进行了详细表征。通过体内和体外螺旋计算机断层扫描（HCT）以及体外μCT对相同标本进行相关分析。涎石中的矿物质主要由磷灰石（89体积%）和白磷钙矿（11体积%）组成，平均密度为1.8 g/cm³。在水合条件下，矿物质质量占主导，为53±13 wt%，而密度为1.2 g/cm³的有机物占涎石体积的65±10%。针对HCT和μCT都提出了涎石矿物质密度与X射线衰减之间的定量关系。
